目的 了解細(xì)胞增殖標(biāo)記物微小染色體支持蛋白2（minichromosome maintenance protein 2，MCM2）在大腸腫瘤診斷中的應(yīng)用現(xiàn)狀。 方法復(fù)習(xí)國(guó)內(nèi)、外的文獻(xiàn)并加以綜述。結(jié)果 MCM2在腫瘤研究中可作為評(píng)估其細(xì)胞分化發(fā)育異常和突變的指標(biāo)，細(xì)致地分析MCM2在不同大腸細(xì)胞突變階段的表達(dá)有助于早期監(jiān)測(cè)大腸細(xì)胞的突變。結(jié)論MCM2作為一個(gè)相對(duì)敏感而特異性的細(xì)胞增殖標(biāo)記物，有望為早期臨床檢測(cè)大腸癌提供一種新的指標(biāo)，從而有助于早期診斷大腸癌，提高患者的治愈率。
目的 探討雙水平氣道正壓通氣( BiPAP) 呼吸機(jī)加平臺(tái)呼氣閥( PEV) 聯(lián)合呼吸興奮劑治療慢性阻塞性肺疾病急性加重( AECOPD) 合并肺性腦病的療效。方法 70 例AECOPD 合并肺性腦病的患者隨機(jī)分為治療組和對(duì)照組。兩組均予以抗感染、解痙平喘、止咳祛痰等常規(guī)治療, 根據(jù)痰培養(yǎng)及藥敏結(jié)果應(yīng)用敏感抗生素, 并使用BiPAP 呼吸機(jī)治療。治療組在此基礎(chǔ)上加PEV, 并加用納洛酮和尼可剎米靜滴治療( 療程3 d) 。觀察兩組神志轉(zhuǎn)清時(shí)間、生命體征、動(dòng)脈血?dú)狻PACHEⅡ評(píng)分及不良反應(yīng)。結(jié)果 治療組與對(duì)照組相比, 治療后心率、呼吸頻率、PaCO2 及APACHEⅡ評(píng)分均明顯下降, 神志轉(zhuǎn)清時(shí)間縮短, PaO2 、SaO2 、pH 值及格拉斯哥昏迷評(píng)分明顯上升, 差異均具有統(tǒng)計(jì)學(xué)意義( P lt;0. 01) 。治療組2 例因療效不佳行氣管插管機(jī)械通氣, 死亡1 例。對(duì)照組5 例因療效不佳行氣管插管機(jī)械通氣, 死亡2 例。結(jié)論 早期使用BiPAP 呼吸機(jī)加PEV 聯(lián)合呼吸興奮劑治療AECOPD合并肺性腦病患者, 能明顯改善癥狀, 縮短神志轉(zhuǎn)清時(shí)間, 減少氣管插管, 迅速糾正低氧血癥和CO2 潴留, 療效顯著。
【摘要】 目的 探討微弧氧化（microarc oxidation，MAO）結(jié)合應(yīng)用于純鈦種植體表面處理的可行性?！》椒ā「鶕?jù)對(duì)純鈦鈦片處理的不同將實(shí)驗(yàn)分為對(duì)照組（A組，不作處理）、MAO組（B組，純鈦片上進(jìn)行MAO處理）及MAO加Ⅰ型膠原組（C組，純鈦片上MAO處理后吸附Ⅰ型膠原）。將成骨細(xì)胞培養(yǎng)于各組鈦片上，通過(guò)掃描電鏡、MTT法檢測(cè)不同時(shí)間點(diǎn)各組鈦片表面的細(xì)胞生長(zhǎng)及增殖情況，并檢測(cè)堿性磷酸酶（alkaline phosphatase，ALP）活性?！〗Y(jié)果 掃描電鏡顯示成骨細(xì)胞在C組鈦片上細(xì)胞黏附情況優(yōu)于A、B組；MTT法及ALP活性檢測(cè)示培養(yǎng)3、6 d，成骨細(xì)胞在C組鈦片上的增殖及ALP活性與A、B組比較差異均有統(tǒng)計(jì)學(xué)意義（Plt;0.05）。 結(jié)論 MAO結(jié)合Ⅰ型膠原處理的鈦片可更有效提高成骨細(xì)胞表面附著、增殖，且具有較高的ALP活性。【Abstract】 Objective To study the feasibility of applying microarc oxidation (MAO) with collagen Ⅰ in surface modification of pure titanium. Methods According to different processing methods, the pure titanium was divided into three groups: the control group (without surface modification, group A), MAO group (with microarc oxidation applied in pure titanium surface modification, group B), MAO+Ⅰ group (with microarc oxidation and collagen Ⅰ applied in pure titanium treatment, group C). Osteoblasts were cultured on the surface of titanium in each group, and the cell proliferation in each group was detected at different time points by scanning electron microscopy and MTT method. Moreover, the activity of alkaline phosphatase (ALP) was also detected. Results Scanning electron microscopy showed that adhesion of osteoblasts for group C was better than group A and group B. MTT method and ALP activity detection indicated that there was a significant difference between group C and group A, B in cell proliferation and ALP activity on the third and sixth day of cultivation (Plt;0.05). Conclusion MAO with collagen Ⅰ applied in surface modification of pure titanium may increase osteoblast attachment, and promote its proliferation and ALP activity.
【摘要】 目的 探討凋亡抑制蛋白Livin與凋亡蛋白Caspase-3在結(jié)直腸腺瘤-癌序列中的表達(dá)變化及其相關(guān)性。 方法 2006年7月—2009年12月，采用免疫組織化學(xué)染色鏈霉菌抗生物素蛋白-過(guò)氧化物酶鏈接法streptavidin-peroxidese，SP）法檢測(cè)18例正常黏膜、84例結(jié)直腸腺瘤、72例結(jié)直腸癌中Livin及Caspase-3的表達(dá)情況。 結(jié)果 結(jié)直腸腺瘤組織中Livin蛋白的陽(yáng)性表達(dá)率明顯高于正常黏膜組織（Plt;0.05），而低于腺癌組（Plt;0.05）；腺瘤組內(nèi)絨毛狀腺瘤與管狀腺瘤相比較，Livin蛋白表達(dá)率差異有統(tǒng)計(jì)學(xué)意義（Plt;0.05）。結(jié)直腸腺瘤組織中Caspase-3的陽(yáng)性表達(dá)率明顯高于正常黏膜組織（Plt;0.05）；而腺瘤組織與癌組織之間Caspase-3陽(yáng)性表達(dá)率差異（Plt;0.05）；腺瘤組內(nèi)絨毛狀腺瘤與管狀腺瘤相比較，Caspase-3蛋白陽(yáng)性表達(dá)率差異無(wú)統(tǒng)計(jì)學(xué)意義（Pgt;0.05）。Livin表達(dá)與Caspase-3表達(dá)呈負(fù)相關(guān)（Plt;0.05）?！〗Y(jié)論 凋亡抑制蛋白Livin參與了大腸腫瘤的發(fā)生，且在大腸腺瘤-腺癌階段起到了重要作用；凋亡抑制蛋白Livin與Caspase-3表達(dá)呈負(fù)相關(guān)，抑制Caspase-3蛋白的活性可能是Livin促進(jìn)結(jié)腸癌發(fā)生的途徑之一?！続bstract】 Objective To investigate the expression of Livin and Caspase-3 among colorectal adenoma-carcinoma sequence, and to identify the relationship between Livin and Caspase-3 expression in colorectal adenoma-carcinoma sequence. Methods Formalin-fixed paraffin embedded colorectal tissues from 174 patients, including 84 adenomas, 72 carcinomas, and 18 normal mucosa, were examined for expression of Livin and Caspase-3 by streptavidin-peroxidase (SP) immunohistochemistry between July 2006 and December 2009. Results The positive rates of Livin protein expression in colorectal adenoma was significantly higher than that in normal mucosa (Plt;0.05), but lower than that in adenocarcinoma (Plt;0.05); the expression of Livin in tubular adenoma was significantly higher than that in villous adenoma (Plt;0.05). The positive rates of Caspase-3 protein expression in colorectal adenoma were significantly higher than that in normal mucosa and carcinoma (Plt;0.05), and the difference in positive rate of Caspase-3 expression was not significant between the villous adenoma and tubular adenoma (Pgt;0.05). Livin expression had negative correlation with the Caspase-3 expression (Pgt;0.05). Conclusion The difference in expression of Livin between adenoma and adenocarcinoma indicates the potential value of it in carcinogenesis of colorectal cancer, which suggestes that suppressing Caspase-3 protein activity is one of the channels by which livin promotes colorectal carcinogenesis.
目的探討人急性胰腺炎早期外周血單核細(xì)胞(peripheral blood monocytes,PBMCs)表面Toll樣受體4(Toll like receptor 4,TLR4)表達(dá)變化。方法發(fā)病24 h內(nèi)入院的早期急性胰腺炎患者30例，采集入院當(dāng)天及第3、7 d外周血及20名正常志愿者外周血，分離單核細(xì)胞。用流式細(xì)胞儀檢測(cè)單核細(xì)胞表面TLR4、CD14表達(dá)變化情況。同時(shí)檢測(cè)血清腫瘤壞死因子-α（tumor necrosis factor alpha,TNF-α）、白細(xì)胞介素6（interleukin 6,IL-6）等指標(biāo)，研究它們之間的相關(guān)性。結(jié)果急性胰腺炎患者PBMCs表面TLR4發(fā)病后表達(dá)上調(diào)， 1周左右恢復(fù)正常。TNF-α的變化與TLR4變化一致。結(jié)論人急性胰腺炎早期可能通過(guò)先天性免疫門(mén)戶蛋白TLR4激活單核巨噬細(xì)胞系統(tǒng)導(dǎo)致TNF-α等促炎細(xì)胞因子的產(chǎn)生和釋放。