【摘要】 目的 制備小鼠腎炎模型并觀察雙氫青蒿素（dihydroartemisinine，DHA）對模型小鼠細胞因子腫瘤壞死因子-α（tunor necrosis factor，TNF-α）和白細胞介素-6（inter leukin-6，IL-6）的影響以及小鼠腎臟的病理變化?！》椒ā∪⌒坌岳ッ鞣N小鼠120只， 隨機分為正常對照組、脂多糖（lipopolysaccharides，LPS）組、LPS+腎勻漿組及DHA治療組；分別于12、24、48 h取血，酶聯(lián)免疫吸附試驗檢測血清中TNF-α和IL-6的含量，蘇木精-伊紅染色法觀察小鼠腎臟的病理變化。 結果 造模48 h LPS+腎勻漿組小鼠腎小球出現(xiàn)炎性細胞浸潤，而正常對照組未見異常；LPS組及 DHA治療組僅有輕微的病理改變。LPS刺激使小鼠血清TNF-α和IL-6含量高于正常水平（Plt;0.01），但有隨時間不斷下降的趨勢；LPS+腎勻漿組較正常對照組TNF-α和IL-6含量升高（Plt;0.01）；DHA可顯著下調模型小鼠血清TNF-α的水平（Plt;0.01），但對IL-6的影響相對較小（Pgt;0.05）?！〗Y論 運用改良的造模方法LPS+腎勻漿建立腎炎模型效果良好；DHA可以調節(jié)模型小鼠炎癥因子TNF-α和IL-6的釋放，具有一定的改善模型小鼠腎炎癥狀的作用。【Abstract】 Objective To establish mice nephritis models, detect the serum level changes of cytokines tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) in the model mice treated by Dihydroartemisinin (DHA), and observe the physiological changes of the mice kidneys. Methods One hundred and twenty male Kunming mice were randomly divided into 4 groups: control group, lipopolysaccharides (LPS) group, LPS plus kidney homogenate group, and DHA treated group. The level of cytokines TNF-α and IL-6 in the serum were detected by enzyme-linked immunoabsordent assey at hour 12, 24, and 48, respectively. Pathological changes were observed by hematoxylin: eosin staining. Results At the time hour 48 after the establishment of the model, inflammatory cell infiltration was observed in the glomerulus of the LPS plus kidney homogenate group, but no abnormality was found in the control group. There were only slight pathological changes in mice models of the LPS group and the DHA treated group. The serum level of TNF-α and IL-6 increased remarkably after the treatment of LPS (Plt;0.01), but declined as time went by. The level of TNF-α and IL-6 increased significantly in LPS plus kidney homogenate group compared with the control group (Plt;0.01). DHA could significantly decrease the TNF-α level in the serum (Plt;0.01), but had a low influence on IL-6 (Pgt;0.05). Conclusion The modified LPS plus kidney homogenate has a good result in model establishing. DHA can regulate the release of TNF-α and IL-6 in the model mice, and may have certain good effects on ameliorating the nephritis pathological changes.