【摘要】 目的 研究腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體（TRAIL）蛋白對SKOV3移植瘤細(xì)胞半胱天冬氨酸蛋白酶-3（Caspase-3）表達的影響及其與腫瘤細(xì)胞凋亡的關(guān)系。 方法 建立雌性裸小鼠SKOV3移植瘤24只，隨機分為4組，每組6只。TRAIL組單用重組人TRAIL蛋白（10 μg/kg），順鉑（DDP）組單用DDP（3 mg/kg），TRAIL+DDP組聯(lián)合使用TRAIL蛋白（10 μg/kg）和DDP（3 mg/kg），空白對照組給予0.5 mL生理鹽水。經(jīng)處理后，各組的組織切片用免疫組織化學(xué)染色檢測Caspase-3的表達和末端脫氧核苷酸轉(zhuǎn)移酶介導(dǎo)核苷酸缺口標(biāo)記技術(shù)（TUNEL）檢測腫瘤細(xì)胞凋亡指數(shù)?！〗Y(jié)果 Caspase-3的表達水平在TRAIL組（171.67±14.38）、DDP組（172.50±14.75）、聯(lián)合組（230.00±40.99）中均明顯高于對照組（135.83±16.25）（Plt;0.05）。SKOV3移植瘤細(xì)胞凋亡指數(shù)在空白對照組、TRAIL組、DDP組和聯(lián)合組分別為16.67±5.43、33.17±8.42、24.33±4.59和40.50±6.16，TRAIL組和聯(lián)合組細(xì)胞凋亡指數(shù)較空白對照組和DDP組明顯增高（Plt;0.05）?！〗Y(jié)論 TRAIL蛋白使卵巢癌移植瘤細(xì)胞的Caspase-3表達增強，TRAIL蛋白促進腫瘤細(xì)胞凋亡發(fā)生?！続bstract】 Objective To investigate the effects of Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on the expression of Cysteine/aspartic acid specific protease- 3 (Caspase-3) in SKOV3 ovarian tumor cells and its relationship with the apoptosis of the ovarian tumor xenografts in nude mice. Methods Twenty-four nude mice with SKOV3 cell line ovarian tumor were randomly divided into four groups with 6 in each group. TRAIL (10 μg/kg) was given to the mice in the TRAIL group; DDP (3 μg/kg) was given to the mice in the DDP group; TRAIL (10 μg/kg) and DDP (3 μg/kg) were given to the mice in the TRAIL+DDP group; and 0.5 mL of saline solution was give to the mice in the control group. The expression of Caspase-3 was detected with immunohistochemistry. The apoptosis index (AI) of cells was determined by Terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling (TUNEL). Results The expression of Caspase-3 in the TRAIL group (171.67±14.38), DDP group (172.50±14.75), and TRAIL+DDP group (230.00±40.99) was significantly higher than that in the control group (135.83±16.25) (Plt;0.05). The apoptosis index for the control group, TRAIL group, DDP group and TRAIL+DDP group was 16.67±5.43, 33.17±8.42, 24.33±4.59, and 40.50±6.16, respectively. The apoptosis index for the TRAIL group and the TRAIL+DDP group was significantly higher than that in the control group and the DDP group (Plt;0.05). Conclusion Soluble TRAIL has an effect on enhancing the expression of Caspase-3 in implanted tumor in nude mice. TRAIL protein can inhibit the growth of SKOV3 cells in nude mice by inducing cell apoptosis.