• 1.蘭州大學第二醫(yī)院急救中心（蘭州730030）;;
  • 2.蘭州大學第二醫(yī)院普外科;;
  • 3.蘭州大學第二醫(yī)院骨科;

【摘要】目的 探討重癥急性胰腺炎（SAP）中IL-10及IL-18的表達及其與SAP肺損傷的關系。
方法 將48只SD大鼠按隨機數(shù)字表法均分為對照組和SAP組，動態(tài)定量測定肺濕重系數(shù)、腹水量、血清淀粉酶、血清IL-10及IL-18的變化和半定量RT-PCR檢測肺組織中IL-10 mRNA及IL-18 mRNA的表達，并在光鏡下觀察胰腺及肺組織病理學改變。
結(jié)果 各時相SAP組肺濕重系數(shù)、腹水量、血清淀粉酶、血清IL-10及IL-18水平和肺組織中IL-10 mRNA及IL-18 mRNA的表達與對照組相比均明顯增高（P＜0.05,P＜0.01）。SAP組大鼠血清中IL-18水平和肺組織IL-18 mRNA表達水平均與肺濕重系數(shù)（r=0.68，P＜0.01； r=0.72，P＜0.01）及肺損傷病理評分（r=0.74,P＜0.01; r=0.79,P＜0.01）呈正相關，而血清中IL-10水平和肺組織IL-10 mRNA表達水平均與肺濕重系數(shù)（r=-0.62, P＜0.01; r=-0.69, P＜0.01)及肺損傷病理評分（r=-0.66,P＜0.01; r=-0.60,P＜0.01）呈負相關。
結(jié)論 IL-18參與了SAP并發(fā)肺損傷的病理過程，而IL-10對肺損傷有保護作用。

引用本文： 王先坤,詹樂恒,王世文,張有成,汪玉良,康迎新. IL-10、IL-18在重癥急性胰腺炎肺損傷中的作用. 中國普外基礎與臨床雜志, 2005, 12(5): 443-446. doi: 復制

版權(quán)信息： ?四川大學華西醫(yī)院華西期刊社《中國普外基礎與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Shields CJ, Winter DC, Redmond HP. Lung injury in acute pancreatitis: mechanisms, prevention, and therapy [Ｊ]. Curr Opin Crit Care， 2002; 8(2)∶158.
2. Kusske AM, Rongione AJ, Ashley SW, et al. Interleukin10 prevents death in lethal necrotizing pancreatitis in mice [Ｊ]. Surgery, 1996; 120(2)∶284.
3. Denham W, Yang J, Fink G, et al. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis [Ｊ]. Gastroenterology, 1997; 113(5)∶1741.
4. Hofbauer B, Saluja AK, Bhatia M, et al. Effect of recombinant plateletactivating factor acetylhydrolase on two models of experimental acute pancreatitis [Ｊ]. Gastroenterology, 1998; 115(5)∶1238.
5. Norman J. The role of cytokines in the pathogenesis of acute pancreatitis [Ｊ]. Am J Surg, 1998； 175(1)∶76.
6. Leung BP, Culshaw S, Gracie JA, et al. A role for IL18 in neutrophil activation [Ｊ]. J Immunol, 2001; 167(5)∶2879.
7. Dinarello CA. IL18: A TH1inducing, proinflammatory cytokine and new member of the IL1 family [Ｊ]. J Allergy Clin Immunol, 1999; 103(1 Pt 1)∶11.
8. Jordan JA, Guo RF, Yun EC, et al. Role of IL18 in acute lung inflammation [Ｊ]. J Immunol, 2001; 167(12)∶7060.
9. Imrie CW, McKay CJ. The scientific basis of medical therapy of acute pancreatitis. Could it work, and is there a role for lexipafant? [Ｊ]. Gastroenterol Clin North Am, 1999; 28(3)∶591.
10. WereszczynskaSiemiatkowska U, Dabrowski A, Siemiatkowski A, et al. Serum profiles of Eselectin, interleukin10, and interleukin6 and oxidative stress parameters in patients with acute pancreatitis and nonpancreatic acute abdominal pain [Ｊ]. Pancreas, 2003; 26(2)∶144.
  1. 1. Shields CJ, Winter DC, Redmond HP. Lung injury in acute pancreatitis: mechanisms, prevention, and therapy [Ｊ]. Curr Opin Crit Care， 2002; 8(2)∶158.
  2. 2. Kusske AM, Rongione AJ, Ashley SW, et al. Interleukin10 prevents death in lethal necrotizing pancreatitis in mice [Ｊ]. Surgery, 1996; 120(2)∶284.
  3. 3. Denham W, Yang J, Fink G, et al. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis [Ｊ]. Gastroenterology, 1997; 113(5)∶1741.
  4. 4. Hofbauer B, Saluja AK, Bhatia M, et al. Effect of recombinant plateletactivating factor acetylhydrolase on two models of experimental acute pancreatitis [Ｊ]. Gastroenterology, 1998; 115(5)∶1238.
  5. 5. Norman J. The role of cytokines in the pathogenesis of acute pancreatitis [Ｊ]. Am J Surg, 1998； 175(1)∶76.
  6. 6. Leung BP, Culshaw S, Gracie JA, et al. A role for IL18 in neutrophil activation [Ｊ]. J Immunol, 2001; 167(5)∶2879.
  7. 7. Dinarello CA. IL18: A TH1inducing, proinflammatory cytokine and new member of the IL1 family [Ｊ]. J Allergy Clin Immunol, 1999; 103(1 Pt 1)∶11.
  8. 8. Jordan JA, Guo RF, Yun EC, et al. Role of IL18 in acute lung inflammation [Ｊ]. J Immunol, 2001; 167(12)∶7060.
  9. 9. Imrie CW, McKay CJ. The scientific basis of medical therapy of acute pancreatitis. Could it work, and is there a role for lexipafant? [Ｊ]. Gastroenterol Clin North Am, 1999; 28(3)∶591.
  10. 10. WereszczynskaSiemiatkowska U, Dabrowski A, Siemiatkowski A, et al. Serum profiles of Eselectin, interleukin10, and interleukin6 and oxidative stress parameters in patients with acute pancreatitis and nonpancreatic acute abdominal pain [Ｊ]. Pancreas, 2003; 26(2)∶144.