• 中南大學(xué)湘雅二醫(yī)院老年外科（長(zhǎng)沙410011）;

【摘要】目的 探討生長(zhǎng)激素（GH）對(duì)結(jié)腸癌細(xì)胞的作用，為其臨床應(yīng)用的安全性研究提供實(shí)驗(yàn)依據(jù)。
方法 建立人結(jié)腸癌細(xì)胞株SW480裸鼠移植瘤模型，觀察GH對(duì)移植瘤的作用； 5-FU和GH聯(lián)合干預(yù)體外培養(yǎng)SW480細(xì)胞株，通過(guò)MTT法觀察細(xì)胞生長(zhǎng)變化。
結(jié)果 GH組移植瘤的體積、平均直徑及重量均顯著高于對(duì)照組（P＜0.05）； 通過(guò)MTT法觀察到GH組A值顯著高于對(duì)照組（P＜0.01），而5-FU與GH聯(lián)合干預(yù)組則明顯低于對(duì)照組（P＜0.01）。
結(jié)論 GH對(duì)結(jié)腸癌細(xì)胞具有顯著的促生長(zhǎng)作用； GH與細(xì)胞周期特異性化療藥物的聯(lián)合應(yīng)用具有一定安全性，并有可能成為化療增敏劑。

引用本文： 周建平,劉祺. 生長(zhǎng)激素對(duì)人結(jié)腸癌細(xì)胞作用的研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(4): 390-392. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Swerdlow AJ, Higgins CD, Adlard P，et al. Risk of cancer in patients treated with human pituitary growth hormone in the UK, 1959-85: a cohort study [Ｊ]. JPLancet, 2002; 360(9329)∶273.
2. Jauch KW, Heiss MM, Gruetzner U, et al. Prognostic significance of bone marrow micrometastases in patients with gastric cancer [Ｊ]. J Clin Oncol, 1996; 14(6)∶1810.
3. Hermanek P. pTNM and residual tumor classifications: problems of assessment and prognostic significance [Ｊ]. World J Surg, 1995; 19(2)∶184.
4. Cohen P, Clemmons DR, Rosenfeld RG. Dose the GHIGF axis play a role in cancer pathogenesis? [Ｊ]. Growth Horm IGF Res, 2000; 10(6)∶297.
5. Morante J, VallejoCremades MT, GomezGarcia L, et al. Differential action of growth hormone in irradiated tumoral and nontumoral intestinal tissue [Ｊ]. Dig Dis Sci, 2003; 48(11)∶2159.
6. Bustin SA, Jenkins PJ. The growth hormoneinsulinlike growth factorI axis and colorectal cancer [Ｊ]. Trends Mol Med, 2001; 7(10)∶447.
7. Busto R, Schally AV, Varga JL, et al. The expression of growth hormonereleasing hormone (GHRH) and splice variants of its receptor in human gastroenteropancreatic carcinomas [Ｊ]. Proc Natl Acad Sci USA, 2002; 99(18)∶11866.
8. 祁曉平，黎介壽，陳徹. 人生長(zhǎng)激素對(duì)人結(jié)腸癌細(xì)胞LS174T增殖周期的影響 [Ｊ]. 腸外與腸內(nèi)營(yíng)養(yǎng)， 2001; 8(1)∶8.
9. 祁曉平，黎介壽，陳 徹. 人生長(zhǎng)激素對(duì)結(jié)腸癌細(xì)胞株細(xì)胞周期動(dòng)力學(xué)的影響 [Ｊ]. 腸外與腸內(nèi)營(yíng)養(yǎng)， 2000; 7(2)∶83.
10. 陳嘉勇，甘平，徐鵬遠(yuǎn)，等. 生長(zhǎng)激素對(duì)老年消化道腫瘤患者術(shù)后蛋白分解代謝和免疫功能的影響 [Ｊ]. 中國(guó)老年學(xué)雜志， 2000; 20(5)∶162.
  1. 1. Swerdlow AJ, Higgins CD, Adlard P，et al. Risk of cancer in patients treated with human pituitary growth hormone in the UK, 1959-85: a cohort study [Ｊ]. JPLancet, 2002; 360(9329)∶273.
  2. 2. Jauch KW, Heiss MM, Gruetzner U, et al. Prognostic significance of bone marrow micrometastases in patients with gastric cancer [Ｊ]. J Clin Oncol, 1996; 14(6)∶1810.
  3. 3. Hermanek P. pTNM and residual tumor classifications: problems of assessment and prognostic significance [Ｊ]. World J Surg, 1995; 19(2)∶184.
  4. 4. Cohen P, Clemmons DR, Rosenfeld RG. Dose the GHIGF axis play a role in cancer pathogenesis? [Ｊ]. Growth Horm IGF Res, 2000; 10(6)∶297.
  5. 5. Morante J, VallejoCremades MT, GomezGarcia L, et al. Differential action of growth hormone in irradiated tumoral and nontumoral intestinal tissue [Ｊ]. Dig Dis Sci, 2003; 48(11)∶2159.
  6. 6. Bustin SA, Jenkins PJ. The growth hormoneinsulinlike growth factorI axis and colorectal cancer [Ｊ]. Trends Mol Med, 2001; 7(10)∶447.
  7. 7. Busto R, Schally AV, Varga JL, et al. The expression of growth hormonereleasing hormone (GHRH) and splice variants of its receptor in human gastroenteropancreatic carcinomas [Ｊ]. Proc Natl Acad Sci USA, 2002; 99(18)∶11866.
  8. 8. 祁曉平，黎介壽，陳徹. 人生長(zhǎng)激素對(duì)人結(jié)腸癌細(xì)胞LS174T增殖周期的影響 [Ｊ]. 腸外與腸內(nèi)營(yíng)養(yǎng)， 2001; 8(1)∶8.
  9. 9. 祁曉平，黎介壽，陳 徹. 人生長(zhǎng)激素對(duì)結(jié)腸癌細(xì)胞株細(xì)胞周期動(dòng)力學(xué)的影響 [Ｊ]. 腸外與腸內(nèi)營(yíng)養(yǎng)， 2000; 7(2)∶83.
  10. 10. 陳嘉勇，甘平，徐鵬遠(yuǎn)，等. 生長(zhǎng)激素對(duì)老年消化道腫瘤患者術(shù)后蛋白分解代謝和免疫功能的影響 [Ｊ]. 中國(guó)老年學(xué)雜志， 2000; 20(5)∶162.