陳波 1 , 陸云飛 2 , 曾健 2 , 何生 1
  • 1.四川大學華西醫(yī)院普外科（成都610041）;;
  • 2.廣西醫(yī)科大學第一附屬醫(yī)院普外科（南寧530021）;

【摘要】目的探討檢測乳腺癌腋窩淋巴結轉移較敏感的方法。
方法應用常規(guī)HE染色法、細胞角蛋白19（CK19）作為單抗的免疫組化（IHC）SP染色及CK19 mRNA的逆轉錄聚合酶鏈反應（RT-PCR）3種方法檢測20例乳腺癌患者共239個腋窩淋巴結的轉移情況。
結果239個腋窩淋巴結中，HE染色發(fā)現(xiàn)3例患者7個（2.9%，7/239）有癌轉移，均位于level Ⅰ； IHC檢測到7例患者13個（5.4%，13/239）有癌轉移，其中11個位于level Ⅰ，2個位于level Ⅱ； RT-PCR檢測到14例患者52個（21.8%，52/239）有癌轉移，其中30個位于level Ⅰ，22個位于level Ⅱ。IHC和RT-PCR檢測證實，HE染色陽性的7個淋巴結均有癌轉移； IHC染色陽性的淋巴結均出現(xiàn)了PCR陽性擴增產物。HE、IHC及RT-PCR 3種檢測方法對腋窩淋巴結微轉移的檢出率分別是0(0/232)、2.6%(6/232)及19.4%(45/232），P＜0.05。
結論 IHC及RT-PCR是較HE檢測淋巴結微轉移更敏感的方法，而RT-PCR較IHC更為敏感，能更準確地反映乳腺癌患者腋窩淋巴結的狀況。

引用本文： 陳波,陸云飛,曾健,何生. HE、IHC和RTPCR方法檢測乳腺癌腋窩淋巴結轉移的對照研究. 中國普外基礎與臨床雜志, 2005, 12(3): 238-240. doi: 復制

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  4. 4. International(Ludwig)Breast Cancer Study Group.Prognostic importance of occult axillary lymph node micrometastases from breast cancer [Ｊ]. Lancet, 1990; 335(8705)∶1565.
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  6. 6. Silverstein MJ, Gierson ED, Waisman JR, et al. Axillary lymph node dissection for T1a breast carcinoma. Is it indicated? [Ｊ]. Cancer, 1994; 73(3)∶664.
  7. 7. Giuliano AE, Kirgan DM, Guenther JM, et al. Lymphatic mapping and sentinel lymphadenectomy for breast cancer [Ｊ]. Ann Surg, 1994; 220(3)∶391.