• 南京醫(yī)科大學附屬第一醫(yī)院肝移植中心（南京210029）;;
  • 通訊作者： 李相成;

目的　觀察缺血預處理對大鼠肝臟移植后再灌注早期核轉錄因子-κB(NF-κB)活性和TNF-α、細胞間黏附分子-1(ICAM-1)表達的影響，探討缺血預處理的保護機理。方法　建立大鼠肝臟移植模型，供肝在林格液中保存2 h，實驗分假手術組、對照組和缺血預處理組（IP組）3組，IP組于供肝切取前夾閉第一肝門10 min，然后開放10 min。分別于移植再灌注后 1、2、4及6 h抽血進行肝酶學檢查，切取肝臟作NF-κB活性、TNF-α和ICAM-1表達的測定。結果　IP組肝功能得到有效改善。與假手術組比較，對照組及IP組再灌注后移植物的NF-κB活性明顯增強，且在1、2 h達高峰， 4 h后減弱，TNF-α和ICAM-1表達也隨之增加； 同對照組相比，IP組的NF-κB活性降低，并且在1、2 h差異具有統(tǒng)計學意義（P＜0.05）, TNF-α和ICAM-1表達亦降低。結論　缺血預處理對于供肝的保護作用可能是通過抑制再灌注早期NF-κB的活性，減少了TNF-α和ICAM-1炎癥介質的釋放，從而減輕了移植物再灌注早期的炎癥反應實現的。

引用本文： 馬躍峰,李相成,姚愛華,游偉,王學浩. 缺血預處理對大鼠肝臟移植物再灌注早期NF-κB活性的影響及意義△. 中國普外基礎與臨床雜志, 2006, 13(5): 531-534. doi: 復制

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2. 　涂　兵， 嚴律南， 劉智敏， 等. 缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用 [J]. 中國普外基礎與臨床雜志， 2002； 9（2）∶89.
3. 　Zhang SJ, Chen S. The role of ischemic preconditioning in rat liver graft [J].Transplant Proc, 2004; 36(7)∶1970.
4. 　馬躍峰， 李相成， 游　偉. “兩袖套法”大鼠原位肝移植模型的技術改進 [J]. 中國現代醫(yī)學雜志， 2006； 16（2）∶229.
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6. 　Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia-reperfusion injury [J]. Am J Surg， 2001; 181(2)∶160.
7. 　Ricciardi R, Kim RD, McDade TP, et al. NF kappa B expression during cold ischemia correlates with postreperfusion graft function [J]. J Surg Res， 2000; 93(1)∶35.
8. 　Sindram D, Rudiger HA, Upadhya AG, et al. Ischemic preconditioning protects against cold ischemic injury through an oxidative stress dependent mechanism [J]. J Hepatol， 2002; 36(1)∶78.
9. 　Nilsson B, Friman S, Wallin M, et al. The liver protective effect of ischemic preconditioning may be mediated by adenosine [J]. Transpl Int， 2000; 13 Suppl 1∶S558.
10. 　Yamamoto Y, Kume M, Yamaoka Y. Implications of heat shock proteins during liver surgery and liver perfusion [J]. Recent Results Cancer Res， 1998; 147∶157.
11. 　Jiang Y, Gu XP, Qiu YD, et al. Ischemic preconditioning decreases C-X-C chemokine expression and neutrophil accumulation early after liver transplantation in rats [J]. World J Gastroenterol， 2003; 9(9)∶2025.
12. 　Tsuyama H, Shimizu K, Yoshimoto K, et al. Protective effect of ischemic preconditioning on hepatic ischemia-reperfusion injury in mice [J]. Transplant Proc， 2000; 32(7)∶2310.
13. 　Ohkita M, Takaoka M, Shiota Y, et al. Nitric oxide inhibits endothelin-1 production through the suppression of nuclear factor kappa B [J]. Clin Sci (Lond)， 2002; 103 Suppl 48∶68S.
14. 　 Rudiger HA, Clavien PA. A cytotoxic drug against reperfusion injury? [J]. Hepatology， 2000; 31(2)∶533.
  1. 1. 　Clavien PA, Harvey PR, Strasberg SM. Preservation and reperfusion injuries in liver allografts. An overview and synthesis of current studies [J]. Transplantation, 1992; 53(5)∶957.
  2. 2. 　涂　兵， 嚴律南， 劉智敏， 等. 缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用 [J]. 中國普外基礎與臨床雜志， 2002； 9（2）∶89.
  3. 3. 　Zhang SJ, Chen S. The role of ischemic preconditioning in rat liver graft [J].Transplant Proc, 2004; 36(7)∶1970.
  4. 4. 　馬躍峰， 李相成， 游　偉. “兩袖套法”大鼠原位肝移植模型的技術改進 [J]. 中國現代醫(yī)學雜志， 2006； 16（2）∶229.
  5. 5. 　 Kataoka M, Shimizu H, Mitsuhashi N, et al. Effect of cold-ischemia time on C-X-C chemokine expression and neutrophil accumulation in the graft liver after orthotopic liver transplantation in rats [J]. Transplantation， 2002; 73(11)∶1730.
  6. 6. 　Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia-reperfusion injury [J]. Am J Surg， 2001; 181(2)∶160.
  7. 7. 　Ricciardi R, Kim RD, McDade TP, et al. NF kappa B expression during cold ischemia correlates with postreperfusion graft function [J]. J Surg Res， 2000; 93(1)∶35.
  8. 8. 　Sindram D, Rudiger HA, Upadhya AG, et al. Ischemic preconditioning protects against cold ischemic injury through an oxidative stress dependent mechanism [J]. J Hepatol， 2002; 36(1)∶78.
  9. 9. 　Nilsson B, Friman S, Wallin M, et al. The liver protective effect of ischemic preconditioning may be mediated by adenosine [J]. Transpl Int， 2000; 13 Suppl 1∶S558.
  10. 10. 　Yamamoto Y, Kume M, Yamaoka Y. Implications of heat shock proteins during liver surgery and liver perfusion [J]. Recent Results Cancer Res， 1998; 147∶157.
  11. 11. 　Jiang Y, Gu XP, Qiu YD, et al. Ischemic preconditioning decreases C-X-C chemokine expression and neutrophil accumulation early after liver transplantation in rats [J]. World J Gastroenterol， 2003; 9(9)∶2025.
  12. 12. 　Tsuyama H, Shimizu K, Yoshimoto K, et al. Protective effect of ischemic preconditioning on hepatic ischemia-reperfusion injury in mice [J]. Transplant Proc， 2000; 32(7)∶2310.
  13. 13. 　Ohkita M, Takaoka M, Shiota Y, et al. Nitric oxide inhibits endothelin-1 production through the suppression of nuclear factor kappa B [J]. Clin Sci (Lond)， 2002; 103 Suppl 48∶68S.
  14. 14. 　 Rudiger HA, Clavien PA. A cytotoxic drug against reperfusion injury? [J]. Hepatology， 2000; 31(2)∶533.