• 1.湖南吉首大學(xué)醫(yī)學(xué)院（吉首 416000）;;
  • 2.中南大學(xué)湘雅二醫(yī)院肝膽研究室（長沙 410011）;

目的研究芳香化酶（aromatase, Arom）和存活素（survivin, Surv）在原發(fā)性肝癌（primary hepatocarcinoma, PHC）中的表達(dá)情況，探討它們間的相互關(guān)系以及與PHC臨床病理特征之間的關(guān)系。
方法應(yīng)用ABC免疫組化染色法對47例PHC手術(shù)切除標(biāo)本切片分別檢測Arom和Surv的表達(dá)，并于高倍鏡下評分。
結(jié)果47例PHC癌組織中Arom和Surv的表達(dá)陽性率和表達(dá)評分均顯著高于癌旁組織： Arom, 40.43% vs 21.38%（P＜0.05）, 1.53±1.69 vs 0.79±1.41 （P＜0.05）; Surv, 63.83% vs 31.91%（ P＜0.01）, 2.40±1.96 vs 1.45±1.80 （P＜0.05）。除Surv在腫塊最大徑＜5 cm的癌組織中的表達(dá)評分（4.00±2.10）顯著高于腫塊最大徑≥5 cm者（2.17±1.86）外（P＜0.05），Arom和Surv在PHC中的表達(dá)情況與PHC其他主要臨床病理特征之間均無明顯關(guān)系； 癌組織中Arom與Surv的表達(dá)評分呈正相關(guān)（r=0.316，P＜0.05）。結(jié)論Arom和Surv的表達(dá)在PHC發(fā)生、發(fā)展中可能有著密切的關(guān)系。

引用本文： 向志鋼,楊竹林,鄧星輝. 原發(fā)性肝癌組織中芳香化酶和存活素的表達(dá)及其臨床病理意義. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(3): 321-324. doi: 復(fù)制

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2. Kappler M, Kotzsch M, Bartel F, et al. Elevated expression level of survivin protein in softtissue sarcomas is a strong independent predictor of survival [Ｊ]. Clin Cancer Res, 2003; 9(3)∶1098.
3. 胡淳玲， 喻倫銀， 陳德基， 等. 大鼠試驗(yàn)性肺鱗癌癌變各階段間質(zhì)微血管密度及VEGF、FLK1表達(dá)的動態(tài)變化 [Ｊ]. 癌癥， 2001; 20(10)∶713.
4. Meinhardt U, Mullis PE. The aromatase cytochrome P450 and its clinical impact [Ｊ]. Horm Res, 2002; 57(5-6)∶145.
5. Castagnetta LA, Agostara B, Montalto G, et al. Local estrogen formation by nontumoral, cirrhotic, and malignant human liver tissues and cells[Ｊ]. Cancer Res, 2003; 63(16)∶5041.
6. Shin S, Sung BJ, Cho YS, et al. An antiapoptotic protein human survivin is a direct inhibitor of caspase3 and 7[Ｊ]. Biochemistry, 2001; 40(4)∶1117.
7. 林木生， 伊新， 繆輝來， 等. 生存素和天冬氨酸特異性半胱氨酸蛋白酶3在肝癌中的表達(dá)及其關(guān)系[Ｊ]. 中華實(shí)驗(yàn)外科雜志, 2004; 21(3)∶210.
8. Shekhar MP, Werdell J, Tait L. Interaction with endothelial cells is a prerequisite for branching ductalalveolar morphogenesis and hyperplasia of preneoplastic human breast epithelial cells: regulation by estrogen[Ｊ]. Cancer Res, 2000;60(2)∶439.
9. Kirma N, Gill K, Mandava U, et al. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice [Ｊ]. Cancer Res, 2001; 61(5)∶1910.
10. Thiantanawat A, Long BJ, Brodie AM. Signaling pathways of apoptosis activated by aromatase inhibitors and antiestrogens[Ｊ]. Cancer Res, 2003; 63(22)∶8037.
  1. 1. Harada N, Ota H, Yoshimura N, et al. Localized aberrant expression of cytochrome P450 aromatase in primary and metastatic malignant tumors of human liver [Ｊ]. J Clin Endocrinol Metab, 1998; 83(2)∶ 697.
  2. 2. Kappler M, Kotzsch M, Bartel F, et al. Elevated expression level of survivin protein in softtissue sarcomas is a strong independent predictor of survival [Ｊ]. Clin Cancer Res, 2003; 9(3)∶1098.
  3. 3. 胡淳玲， 喻倫銀， 陳德基， 等. 大鼠試驗(yàn)性肺鱗癌癌變各階段間質(zhì)微血管密度及VEGF、FLK1表達(dá)的動態(tài)變化 [Ｊ]. 癌癥， 2001; 20(10)∶713.
  4. 4. Meinhardt U, Mullis PE. The aromatase cytochrome P450 and its clinical impact [Ｊ]. Horm Res, 2002; 57(5-6)∶145.
  5. 5. Castagnetta LA, Agostara B, Montalto G, et al. Local estrogen formation by nontumoral, cirrhotic, and malignant human liver tissues and cells[Ｊ]. Cancer Res, 2003; 63(16)∶5041.
  6. 6. Shin S, Sung BJ, Cho YS, et al. An antiapoptotic protein human survivin is a direct inhibitor of caspase3 and 7[Ｊ]. Biochemistry, 2001; 40(4)∶1117.
  7. 7. 林木生， 伊新， 繆輝來， 等. 生存素和天冬氨酸特異性半胱氨酸蛋白酶3在肝癌中的表達(dá)及其關(guān)系[Ｊ]. 中華實(shí)驗(yàn)外科雜志, 2004; 21(3)∶210.
  8. 8. Shekhar MP, Werdell J, Tait L. Interaction with endothelial cells is a prerequisite for branching ductalalveolar morphogenesis and hyperplasia of preneoplastic human breast epithelial cells: regulation by estrogen[Ｊ]. Cancer Res, 2000;60(2)∶439.
  9. 9. Kirma N, Gill K, Mandava U, et al. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice [Ｊ]. Cancer Res, 2001; 61(5)∶1910.
  10. 10. Thiantanawat A, Long BJ, Brodie AM. Signaling pathways of apoptosis activated by aromatase inhibitors and antiestrogens[Ｊ]. Cancer Res, 2003; 63(22)∶8037.