• 1.四川大學(xué)華西醫(yī)院普外二科(成都610041);;
  • 2.大連醫(yī)科大學(xué)附屬第一醫(yī)院(大連116011);;
  • 通訊作者: 嚴(yán)律南;

【摘要】目的探討細(xì)菌性腹膜炎致多器官功能不全綜合征（MODS）大鼠結(jié)腸運(yùn)動(dòng)的變化和細(xì)胞因子白介素6（IL6）、腫瘤壞死因子α（TNFα）及誘生性一氧化氮合成酶（iNOS）對(duì)結(jié)腸運(yùn)動(dòng)的影響。
方法將Wistar大鼠隨機(jī)分成正常對(duì)照組和MODS組； 分別觀測(cè)2組大鼠排便的糞點(diǎn)數(shù)、結(jié)腸肌條的振幅、結(jié)腸平滑肌細(xì)胞長(zhǎng)度以及血清一氧化氮（NO）的變化； 利用免疫組化和逆轉(zhuǎn)錄聚合酶鏈反應(yīng)（RTPCR）方法檢測(cè)結(jié)腸平滑肌IL6、TNFα和iNOS表達(dá)的變化。
結(jié)果MODS組排便的糞點(diǎn)數(shù)和肌條的振幅均明顯低于正常對(duì)照組（P＜0.05）； IL6、TNFα和iNOS蛋白表達(dá)在正常對(duì)照組為陰性表達(dá)，在MODS組為陽性表達(dá)； 在正常對(duì)照組未檢出IL6 mRNA、TNFα mRNA和iNOS mRNA的表達(dá)，而在MODS組，IL6 mRNA、TNFα mRNA和iNOS mRNA均呈陽性表達(dá)； MODS組平滑肌細(xì)胞的長(zhǎng)度和血清NO水平均較正常對(duì)照組明顯增高（P＜0.01）。
結(jié)論MODS后結(jié)腸運(yùn)動(dòng)功能障礙與iNOS、IL6和TNFα密切相關(guān)。

引用本文： 陳哲宇,齊清會(huì),嚴(yán)律南. 細(xì)菌性腹膜炎誘導(dǎo)大鼠多器官功能不全綜合征后結(jié)腸運(yùn)動(dòng)的變化和機(jī)理. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006, 13(3): 287-290. doi: 復(fù)制

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  1. 1. Lodato RF, Khan AR, Zembowicz MJ, et al. Roles of IL1 and TNF in the decreased ileal muscle contractility induced by lipopolysaccharide [Ｊ]. Am J Physiol, 1999; 276(6 Pt 1)∶G1356.
  2. 2. 陳哲宇, 齊清會(huì). 多器官功能不全綜合征大鼠結(jié)腸運(yùn)動(dòng)功能變化和機(jī)制的研究 [Ｊ]. 中華實(shí)驗(yàn)外科雜志, 2004; 21(3)∶341.
  3. 3. Hierholzer C, Kalff JC, Chakraborty A, et al. Impaired gut contractility following hemorrhagic shock is accompaied by IL6 and GCSF production and neutrophil infiltration [Ｊ]. Dig Dis Sci, 2001; 46(2)∶230.
  4. 4. Hierholzer C, Kalff JC, Billiar TR, et al. Induced nitric oxide promotes intestinal inflammation following hemorrhagic shock [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2004; 286(2)∶G225.
  5. 5. De Winter BY. Study of the pathogenesis of paralytic ileus in animal models of experimentally induced postoperative and septic ileus [Ｊ]. Verh K Acad Geneeskd Belg, 2003; 65(5)∶293.
  6. 6. Bani D, Baccari MC, Quattrone S, et al. Relaxin depresses small bowel motility through a nitric oxidemediated mechanism： Studies in mice [Ｊ]. Biol Reprod, 2002; 66(3)∶778.