• 西安交通大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院普通外科（西安710061）;

目的了解上皮型鈣黏附分子（E-cadherin）下調(diào)對(duì)腫瘤細(xì)胞浸潤(rùn)能力的影響。
方法選用人胰腺癌細(xì)胞株JHP-1，經(jīng)E-cadherin反義寡核苷酸（ASODN）作用，應(yīng)用免疫細(xì)胞化學(xué)染色、Western blot法檢測(cè)細(xì)胞E-cadherin蛋白表達(dá)情況，細(xì)胞浸潤(rùn)MTT比色法檢測(cè)該細(xì)胞對(duì)基底膜的浸潤(rùn)能力。
結(jié)果經(jīng)E-cadherin ASODN作用后， JHP-1細(xì)胞膜E-cadherin表達(dá)減弱， E-cadherin蛋白含量與對(duì)照組相比明顯降低； JHP-1細(xì)胞對(duì)基底膜浸潤(rùn)能力明顯高于對(duì)照組（P＜0.001）。
結(jié)論E-cadherin與腫瘤細(xì)胞的浸潤(rùn)能力有關(guān)。

引用本文： 車(chē)向明,王曙逢,樊林,張如愿,趙偉. E-cadherin反義寡核苷酸對(duì)腫瘤細(xì)胞浸潤(rùn)能力影響的研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006, 13(2): 191-193. doi: 復(fù)制

1. Xiangming C, Hokita S, Nuruki K, et al. The expression of cadherincatenin complex in association with the clinicopathologic features of early gastric cancer [Ｊ]. Surg Today, 1998; 28(6)∶ 587.
2. 董全進(jìn)， 鄒壽椿， 鄧高里， 等. 表皮鈣黏附素和α連接素在結(jié)直腸癌中表達(dá)的臨床意義 [Ｊ]. 中華胃腸外科雜志， 2002; 5（4）∶291.
3. Xiangming C, Hokita S, Natsugoe S, et al. Cooccurrence of reduced expression of alphacatenin and overexpression of p53 is a predictor of lymph node metastasis in early gastric cancer [Ｊ]. Oncology, 1999; 57(2)∶131.
4. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding [Ｊ]. Anal Biochem, 1976; 72（1）∶248.
5. Jansen B, SchlagbauerWadl H, Brown BD, et al. bcl2 antisense therapy chemosensitizes human melanoma in SCID mice [Ｊ]. Nat Med, 1998; 4(2)∶232.
6. Imamura H, Takao S, Aikou T. A modified invasion3(4,5dimethylthiazole2yl)2,5diphenyltetrazolium bromide assay for quantitating tumor cell invasion [Ｊ]. Cancer Res, 1994; 54(13)∶3620.
  1. 1. Xiangming C, Hokita S, Nuruki K, et al. The expression of cadherincatenin complex in association with the clinicopathologic features of early gastric cancer [Ｊ]. Surg Today, 1998; 28(6)∶ 587.
  2. 2. 董全進(jìn)， 鄒壽椿， 鄧高里， 等. 表皮鈣黏附素和α連接素在結(jié)直腸癌中表達(dá)的臨床意義 [Ｊ]. 中華胃腸外科雜志， 2002; 5（4）∶291.
  3. 3. Xiangming C, Hokita S, Natsugoe S, et al. Cooccurrence of reduced expression of alphacatenin and overexpression of p53 is a predictor of lymph node metastasis in early gastric cancer [Ｊ]. Oncology, 1999; 57(2)∶131.
  4. 4. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding [Ｊ]. Anal Biochem, 1976; 72（1）∶248.
  5. 5. Jansen B, SchlagbauerWadl H, Brown BD, et al. bcl2 antisense therapy chemosensitizes human melanoma in SCID mice [Ｊ]. Nat Med, 1998; 4(2)∶232.
  6. 6. Imamura H, Takao S, Aikou T. A modified invasion3(4,5dimethylthiazole2yl)2,5diphenyltetrazolium bromide assay for quantitating tumor cell invasion [Ｊ]. Cancer Res, 1994; 54(13)∶3620.