• 中南大學湘雅二醫(yī)院普外科 (長沙410011);

目的 　研究胃癌及其癌旁組織中SKP2和p27蛋白表達水平，探討兩者的相互關(guān)系及其臨床意義。
方法 　49例胃癌及20例癌旁組織常規(guī)制作石蠟包埋切片，SKP2和p27染色方法為SP免疫組化法。
結(jié)果 　胃癌組織中SKP2表達陽性率及其評分值明顯高于癌旁組織(P＜0.01)，而p27則相反(P＜0.05，P＜0.01)； SKP2 表達陽性和(或)p27表達陰性的癌旁組織呈中至重度不典型增生。SKP2和p27蛋白表達與癌細胞浸潤深度、有無淋巴結(jié)轉(zhuǎn)移、淋巴結(jié)轉(zhuǎn)移分站及有無遠處轉(zhuǎn)移有關(guān)(P＜0.05，P＜0.01)。在胃癌組織中SKP2 與p27評分值之間呈負相關(guān)(r=-0.65,P＜0.01)。
結(jié)論 　SKP2和p27可能是反映胃癌發(fā)生、進展、生物學行為及預后的標記物。

引用本文： 姚宏亮,楊竹林,李永國,劉國文. 胃癌及癌旁組織中SKP2 和p27蛋白表達及相互關(guān)系研究. 中國普外基礎(chǔ)與臨床雜志, 2007, 14(3): 308-311. doi: 復制

版權(quán)信息： ?四川大學華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Harada K, Supriatno, Kawashima Y, et al. Down-regulation of S-phase kinase associated protein 2 (Skp2) induces apoptosis in oral cancer cells [J]. Oral Oncol， 2005; 41(6)∶623.
2. Oliveira AM, Okuno SH, Nascimento AG, et al. Skp2 protein expression in soft tissue sarcomas [J]. J Clin Oncol， 2003; 21(4)∶722.
3. Takanami I. The prognostic value of overexpression of Skp2 mRNA in non-small cell lung cancer [J]. Oncol Rep， 2005; 13(4)∶727.
4. Li JQ, Wu F, Masaki T, et al. Correlation of Skp2 with carcinogenesis, invasion, metastasis, and prognosis in colorectal tumors [J]. Int J Oncol， 2004; 25(1)∶87.
5. Shim EH, Johnson L, Noh HL, et al. Expression of the F-box protein SKP2 induces hyperplasia, dysplasia, and low-grade carcinoma in the mouse prostate [J]. Cancer Res， 2003; 63(7)∶1583.
6. Tigli H, Buyru N, Dalay N. Molecular analysis of the p27/kip1 gene in breast cancer [J]. Mol Diagn， 2005; 9(1)∶17.
7. Li Q, Murphy M, Ross J, et al. Skp2 and p27kip1 expression in melanocytic nevi and melanoma: an inverse relationship [J]. J Cutan Pathol， 2004; 31(10)∶633.
8. Fukuchi M, Masuda N, Nakajima M, et al. Inverse correlation between expression levels of p27 and the ubiquitin ligase subunit Skp2 in early esophageal squamous cell carcinoma [J]. Anticancer Res， 2004; 24(2B)∶777.
9. Shintani S, Li C, Mihara M, et al. Skp2 and Jab1 expression are associated with inverse expression of p27(KIP1) and poor prognosis in oral squamous cell carcinomas [J]. Oncology， 2003; 65(4)∶355.
10. 靖昌慶， 李樂平， 李昭. 直腸癌p27基因表達及血清轉(zhuǎn)化生長因子β1水平的變化 [J]. 中國普外基礎(chǔ)與臨床雜志, 2005； 12(1)∶33.
11. 周彥明， 李玉民， 朱有全, 等. cyclin E和p27kip1蛋白在膽囊癌組織中的表達及其意義 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004； 11(2)∶148.
12. Radke S, Pirkmaier A, Germain D. Differential expression of the F-box proteins Skp2 and Skp2B in breast cancer [J]. Oncogene， 2005; 24(21)∶3448.
13. Yokoi S, Yasui K, Mori M, et al. Amplification and overexpression of SKP2 are associated with metastasis of non-small-cell lung cancers to lymph nodes [J]. Am J Pathol， 2004; 165(1)∶175.
14. Langner C, von Wasielewski R, Ratschek M, et al. Expression of p27 and its ubiquitin ligase subunit Skp2 in upper urinary tract transitional cell carcinoma [J]. Urology， 2004; 64(3)∶611.
15. 牛堅， 陳澍周， 錢海鑫. TGFβ1及p27在原發(fā)性膽囊癌中的表達及臨床意義 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004； 11（6）∶518.
  1. 1. Harada K, Supriatno, Kawashima Y, et al. Down-regulation of S-phase kinase associated protein 2 (Skp2) induces apoptosis in oral cancer cells [J]. Oral Oncol， 2005; 41(6)∶623.
  2. 2. Oliveira AM, Okuno SH, Nascimento AG, et al. Skp2 protein expression in soft tissue sarcomas [J]. J Clin Oncol， 2003; 21(4)∶722.
  3. 3. Takanami I. The prognostic value of overexpression of Skp2 mRNA in non-small cell lung cancer [J]. Oncol Rep， 2005; 13(4)∶727.
  4. 4. Li JQ, Wu F, Masaki T, et al. Correlation of Skp2 with carcinogenesis, invasion, metastasis, and prognosis in colorectal tumors [J]. Int J Oncol， 2004; 25(1)∶87.
  5. 5. Shim EH, Johnson L, Noh HL, et al. Expression of the F-box protein SKP2 induces hyperplasia, dysplasia, and low-grade carcinoma in the mouse prostate [J]. Cancer Res， 2003; 63(7)∶1583.
  6. 6. Tigli H, Buyru N, Dalay N. Molecular analysis of the p27/kip1 gene in breast cancer [J]. Mol Diagn， 2005; 9(1)∶17.
  7. 7. Li Q, Murphy M, Ross J, et al. Skp2 and p27kip1 expression in melanocytic nevi and melanoma: an inverse relationship [J]. J Cutan Pathol， 2004; 31(10)∶633.
  8. 8. Fukuchi M, Masuda N, Nakajima M, et al. Inverse correlation between expression levels of p27 and the ubiquitin ligase subunit Skp2 in early esophageal squamous cell carcinoma [J]. Anticancer Res， 2004; 24(2B)∶777.
  9. 9. Shintani S, Li C, Mihara M, et al. Skp2 and Jab1 expression are associated with inverse expression of p27(KIP1) and poor prognosis in oral squamous cell carcinomas [J]. Oncology， 2003; 65(4)∶355.
  10. 10. 靖昌慶， 李樂平， 李昭. 直腸癌p27基因表達及血清轉(zhuǎn)化生長因子β1水平的變化 [J]. 中國普外基礎(chǔ)與臨床雜志, 2005； 12(1)∶33.
  11. 11. 周彥明， 李玉民， 朱有全, 等. cyclin E和p27kip1蛋白在膽囊癌組織中的表達及其意義 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004； 11(2)∶148.
  12. 12. Radke S, Pirkmaier A, Germain D. Differential expression of the F-box proteins Skp2 and Skp2B in breast cancer [J]. Oncogene， 2005; 24(21)∶3448.
  13. 13. Yokoi S, Yasui K, Mori M, et al. Amplification and overexpression of SKP2 are associated with metastasis of non-small-cell lung cancers to lymph nodes [J]. Am J Pathol， 2004; 165(1)∶175.
  14. 14. Langner C, von Wasielewski R, Ratschek M, et al. Expression of p27 and its ubiquitin ligase subunit Skp2 in upper urinary tract transitional cell carcinoma [J]. Urology， 2004; 64(3)∶611.
  15. 15. 牛堅， 陳澍周， 錢海鑫. TGFβ1及p27在原發(fā)性膽囊癌中的表達及臨床意義 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004； 11（6）∶518.