• 第三軍醫(yī)大學(xué)附屬西南醫(yī)院（重慶，400038） 1 關(guān)節(jié)外科中心，2 眼科;

目的 建立保留鈣化層結(jié)構(gòu)的豬股骨滑車全厚軟骨缺損模型，為觀察組織工程軟骨在保留鈣化層
的膝關(guān)節(jié)軟骨缺損模型中的修復(fù)效果提供良好的實(shí)驗(yàn)研究平臺(tái)。 方法 選取6 月齡清潔級(jí)貴州小香豬9 只，體重
40 ～ 50 kg，用標(biāo)準(zhǔn)的軟骨缺損制作套件在其右后肢股骨滑車切跡旁制備直徑6 mm、深0.2 ～ 0.5 mm、不傷及鈣化層結(jié)構(gòu)
的圓柱形全厚軟骨缺損模型。造模4 周后行3.0T MRI 觀察，取材后進(jìn)行大體、體視顯微鏡觀察及固綠- 番紅O、阿利新藍(lán)、
天狼星紅組織學(xué)染色觀察缺損處軟骨修復(fù)情況。 結(jié)果 造模后實(shí)驗(yàn)動(dòng)物均存活，術(shù)后切口無(wú)感染，無(wú)髕骨脫位；術(shù)后即
可下地行走并部分負(fù)重，1 周后均能自由活動(dòng)，無(wú)跛行。造模后4 周，MRI 檢查可見(jiàn)滑車處有明顯連續(xù)信號(hào)中斷，異常信
號(hào)深及軟骨下骨，缺損周邊深層未見(jiàn)明顯信號(hào)異常。標(biāo)本大體觀察示缺損底部有少量填充物、出血點(diǎn)，與周圍正常軟骨界
限清楚。體式顯微鏡觀察示鈣化層基本完整，缺損局部軟骨下骨板有塌陷。普通顯微鏡下，固綠- 番紅O 及阿利新藍(lán)染
色示缺損處無(wú)軟骨細(xì)胞及染料著色；偏光顯微鏡下，天狼星紅染色示缺損底部被連續(xù)、強(qiáng)折光性的纖維組織少量填充。
結(jié)論 通過(guò)該造模方法制作的不傷及鈣化層結(jié)構(gòu)的豬股骨滑車全厚軟骨缺損模型，可用于骨關(guān)節(jié)炎早期軟骨病變修復(fù)的
研究及豬軟骨鈣化層結(jié)構(gòu)作用研究的動(dòng)物模型。

引用本文： 寧志剛 ,楊柳,王富友,張一,王恩洪. 保留鈣化層結(jié)構(gòu)的豬股骨滑車全厚軟骨缺損模型建立. 中國(guó)修復(fù)重建外科雜志, 2012, 26(5): 527-531. doi: 復(fù)制

1. Yokota M, Yasuda K, Kitamura N, et al. Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double-network hydrogel. BMC Musculoskelet Disord, 2011, 12: 49.
2. 代嶺輝, 杜寧. 關(guān)節(jié)軟骨損傷生物學(xué)修復(fù)的研究進(jìn)展. 中國(guó)骨傷, 2009, 22(9): 721-724.
3. Coburn J, Gibson M, Bandalini PA, et al. Biomimetics of the extracellular matrix: an integrated three-dimensional fiber-hydrogel composite for cartilage tissue engineering. Smart Struct Syst, 2011, 7(3): 213-222.
4. Getgood A, Brooks R, Fortier L, et al. Articular cartilage tissue engineering: today’s research, tomorrow’s practice? J Bone Joint Surg (Br), 2009, 91(5): 565-576.
5. Huang GY, Zhou LH, Zhang QC, et al. Microfluidic hydrogels for tissue engineering. Biofabrication, 2011, 3(1): 5087-5092.
6. Chu CR, Szczodyy M, Bruno S. Animal models for cartilage regeneration and repair. Tissue Eng Part B Rev, 2010, 16(1): 105-115.
7. 何志偉, 趙建寧, 岳鵬舉, 等. 可注射型及柱型藻酸鈣載體聯(lián)合運(yùn)用修復(fù)兔膝關(guān)節(jié)軟骨缺損的效果觀察. 中國(guó)骨傷, 2008, 21(12): 899-902.
8. Jiang Y, Chen LK, Zhu DC, et al. The inductive effect of bone morphogenetic protein-4 on chondral-lineage differentiation and in situ cartilage repair. Tissue Eng Part A Rev, 2010, 16(5): 1621-1632.
9. Simon TM, Aberman HM. Cartilage regeneration and repair testing in a surrogate large animal model. Tissue Eng Part B Rev, 2010, 16(1): 65-79.
10. Jung M, Kaszap B, Redöhl A, et al. Enhanced early tissue regeneration after matrix-assisted autologous mesenchymal stem cell transplantation in full thickness chondral defects in a minipig model. Cell Transplant, 2009, 18(8): 923-932.
11. Muehleman C, Li J, Abe Y, et al. Effect of risedronate in a minipig cartilage defect model with allograft. J Orthop Res, 2009, 27(3): 360-365.
12. Nixon AJ, Bequm L, Mohammed HO, et al. Autologous chondrocyte implantation drives early chondrogenesis and organized repair in extensive full- and partial-thickness cartilage defects in an equine model. J Orthop Res, 2011, 29(7): 1121-1130.
13. 王富友, 楊柳, 段小軍, 等. 正常膝關(guān)節(jié)軟骨鈣化層形態(tài)結(jié)構(gòu)研究. 中國(guó)修復(fù)重建外科雜志, 2008, 22(5): 524-527.
14. Madry H, van Dijk CN, Mueller-Gerbl M. The basic science of the subchondral bone. Knee Surg Sports Traumatol Arthrosc, 2010, 18(4): 419-433.
15. Wang F, Ying Z, Duan X, et al. Histomorphometric analysis of adult articular calcified cartilage zone. J Struct Biol, 2009, 168(3): 359-365.
16. Burr DB. Anatomy and physiology of the mineralized tissues: role in the pathogenesis of osteoarthritis. Osteoarthritis Cartilage, 2004, 12 Suppl A: S20-30.
17. Becerra J, Andrades JA, Guerado E, et al. Articular cartilage: structure and rengeneration. Tissue Eng Part B Rev, 2010, 16(6): 617-627.
18. 馬立恒, 劉斯?jié)? 關(guān)節(jié)軟骨的結(jié)構(gòu)與生物力學(xué)的關(guān)系及MR成像. 國(guó)外醫(yī)學(xué): 臨床放射學(xué)分冊(cè), 2006, 29(2): 123-126.
19. Che JH, Zhang ZR, Li GZ, et al. Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits. Knee Surg Sports Traumatol Arthrosc, 2009, 18(4): 496-503.
20. Ahem BJ, Parvizi J, Boston R, et al. Preclinical animal models in single site cartilage defect testing: a systematic review. Osteoarthritis Cartilage, 2009, 17(6): 705-713.
21. Choi JA, Gold GE. MR imaging of articular cartilage physiology. Magn Reson Imaging Clin N Am, 2011, 19(2): 249-282.
22. Cerema MD, Roemer FW, Marra MD, et al. Articular cartilage in the knee: current MR imaging techniques and applications in clinical practice and research. Radiographics, 2011, 31(1): 37-61.
  1. 1. Yokota M, Yasuda K, Kitamura N, et al. Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double-network hydrogel. BMC Musculoskelet Disord, 2011, 12: 49.
  2. 2. 代嶺輝, 杜寧. 關(guān)節(jié)軟骨損傷生物學(xué)修復(fù)的研究進(jìn)展. 中國(guó)骨傷, 2009, 22(9): 721-724.
  3. 3. Coburn J, Gibson M, Bandalini PA, et al. Biomimetics of the extracellular matrix: an integrated three-dimensional fiber-hydrogel composite for cartilage tissue engineering. Smart Struct Syst, 2011, 7(3): 213-222.
  4. 4. Getgood A, Brooks R, Fortier L, et al. Articular cartilage tissue engineering: today’s research, tomorrow’s practice? J Bone Joint Surg (Br), 2009, 91(5): 565-576.
  5. 5. Huang GY, Zhou LH, Zhang QC, et al. Microfluidic hydrogels for tissue engineering. Biofabrication, 2011, 3(1): 5087-5092.
  6. 6. Chu CR, Szczodyy M, Bruno S. Animal models for cartilage regeneration and repair. Tissue Eng Part B Rev, 2010, 16(1): 105-115.
  7. 7. 何志偉, 趙建寧, 岳鵬舉, 等. 可注射型及柱型藻酸鈣載體聯(lián)合運(yùn)用修復(fù)兔膝關(guān)節(jié)軟骨缺損的效果觀察. 中國(guó)骨傷, 2008, 21(12): 899-902.
  8. 8. Jiang Y, Chen LK, Zhu DC, et al. The inductive effect of bone morphogenetic protein-4 on chondral-lineage differentiation and in situ cartilage repair. Tissue Eng Part A Rev, 2010, 16(5): 1621-1632.
  9. 9. Simon TM, Aberman HM. Cartilage regeneration and repair testing in a surrogate large animal model. Tissue Eng Part B Rev, 2010, 16(1): 65-79.
  10. 10. Jung M, Kaszap B, Redöhl A, et al. Enhanced early tissue regeneration after matrix-assisted autologous mesenchymal stem cell transplantation in full thickness chondral defects in a minipig model. Cell Transplant, 2009, 18(8): 923-932.
  11. 11. Muehleman C, Li J, Abe Y, et al. Effect of risedronate in a minipig cartilage defect model with allograft. J Orthop Res, 2009, 27(3): 360-365.
  12. 12. Nixon AJ, Bequm L, Mohammed HO, et al. Autologous chondrocyte implantation drives early chondrogenesis and organized repair in extensive full- and partial-thickness cartilage defects in an equine model. J Orthop Res, 2011, 29(7): 1121-1130.
  13. 13. 王富友, 楊柳, 段小軍, 等. 正常膝關(guān)節(jié)軟骨鈣化層形態(tài)結(jié)構(gòu)研究. 中國(guó)修復(fù)重建外科雜志, 2008, 22(5): 524-527.
  14. 14. Madry H, van Dijk CN, Mueller-Gerbl M. The basic science of the subchondral bone. Knee Surg Sports Traumatol Arthrosc, 2010, 18(4): 419-433.
  15. 15. Wang F, Ying Z, Duan X, et al. Histomorphometric analysis of adult articular calcified cartilage zone. J Struct Biol, 2009, 168(3): 359-365.
  16. 16. Burr DB. Anatomy and physiology of the mineralized tissues: role in the pathogenesis of osteoarthritis. Osteoarthritis Cartilage, 2004, 12 Suppl A: S20-30.
  17. 17. Becerra J, Andrades JA, Guerado E, et al. Articular cartilage: structure and rengeneration. Tissue Eng Part B Rev, 2010, 16(6): 617-627.
  18. 18. 馬立恒, 劉斯?jié)? 關(guān)節(jié)軟骨的結(jié)構(gòu)與生物力學(xué)的關(guān)系及MR成像. 國(guó)外醫(yī)學(xué): 臨床放射學(xué)分冊(cè), 2006, 29(2): 123-126.
  19. 19. Che JH, Zhang ZR, Li GZ, et al. Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits. Knee Surg Sports Traumatol Arthrosc, 2009, 18(4): 496-503.
  20. 20. Ahem BJ, Parvizi J, Boston R, et al. Preclinical animal models in single site cartilage defect testing: a systematic review. Osteoarthritis Cartilage, 2009, 17(6): 705-713.
  21. 21. Choi JA, Gold GE. MR imaging of articular cartilage physiology. Magn Reson Imaging Clin N Am, 2011, 19(2): 249-282.
  22. 22. Cerema MD, Roemer FW, Marra MD, et al. Articular cartilage in the knee: current MR imaging techniques and applications in clinical practice and research. Radiographics, 2011, 31(1): 37-61.