• 1 泰安市中心醫(yī)院呼吸內(nèi)科(山東泰安 271000);;
  • 2 中山大學(xué)附屬第一醫(yī)院呼吸內(nèi)科;

目的  探討水通道蛋白1(AQP-1)在炎癥性胸腔積液大鼠胸膜上的表達(dá)及其與胸水形成的關(guān)系。方法  56只健康Wistar大鼠隨機(jī)分為對照組(8只)和胸膜炎組(48只)。采用角叉菜膠胸腔內(nèi)注射復(fù)制炎癥性胸腔積液大鼠模型。胸膜炎組根據(jù)注入角叉菜膠后處理時間再分為6,12,24,36,48和72 h組,每組8只。測定各組大鼠胸腔積液量,對胸腔積液進(jìn)行細(xì)胞計數(shù)和分類,采用HE染色觀察臟、壁層胸膜的病理變化,采用免疫組化染色檢測AQP-1在大鼠胸膜上的分布,分別采用RT-PCR和Western blot方法檢測臟、壁層胸膜AQP-1的mRNA和蛋白表達(dá)。結(jié)果  胸膜炎各時相組胸水量分別為2.10±0.22、4.10±0.15、4.40±0.36、3.20±0.27、2.60±0.18和0.12±0.02 mL。AQP-1主要表達(dá)在臟、壁層胸膜的間皮細(xì)胞和毛細(xì)血管內(nèi)皮細(xì)胞。胸膜炎各時相組臟、壁層胸膜AQP-1表達(dá)均增強(qiáng)。胸膜炎組AQP-1 mRNA在壁層胸膜的表達(dá)于6 h開始升高,24 h達(dá)高峰;在臟層胸膜的表達(dá)于12 h開始升高,24 h達(dá)高峰。AQP-1蛋白表達(dá)的變化趨勢與mRNA表達(dá)一致。12 h和24 h組壁層胸膜AQP-1表達(dá)與胸腔積液量正相關(guān)(r=0.857,r=0.846,P均 lt;0.01);24 h臟層胸膜AQP-1表達(dá)與胸腔積液量正相關(guān)(r=0.725,P lt;0.05)。結(jié)論  胸膜炎胸腔積液時胸膜AQP-1表達(dá)增強(qiáng),AQP-1可能參與炎癥性胸腔積液的轉(zhuǎn)運過程。

引用本文: 張偉 ,謝燦茂. 角叉菜膠致炎癥性胸腔積液大鼠胸膜上水通道蛋白1的表達(dá). 中國呼吸與危重監(jiān)護(hù)雜志, 2008, 08(3): 190-194. doi: 復(fù)制

1. Light RW,LeeYCG..Textbook of Pleural Diseases.Firsted.Oxford University Press Inc,London,2003.xiii.
2. Manley GT,F(xiàn)ujimura M,Ma T,et al.Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke.Nat Med,2000,6:159-163.
3. Cuzzocrea S,Mazzon E,Calabro G,et al.Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan.Am J Respir Crit Care Med,2000,162:1859-1866.
4. 孫明瑜,謝鳴,張娜,等.小柴胡湯及其藥群配伍對角叉菜膠誘導(dǎo)大鼠胸膜炎模型細(xì)胞因子的影響.中國中西醫(yī)結(jié)合雜志,2004,24:628-631.
5. Corsini E,Di Paola R,Viviani B,Genovese T,Mazzon E,Lucchi L,Marinovich M,Galli CL,Cuzzocrea S.Increased carrageenan-induced acute lung inflammation in old rats.Immunology.2005 Jun;115:253-61.
6. Marzocco S,Di Paola R,Serraino I,et al.Effect of methylguanidine in carrageenan-induced acute inflammation in the rats.Eur J Pharmacol,2004,484:341-350.
7. Sakaguchi Y,Shirahase H,Kunishiro K,et al.Effect of combination of nitric oxide synthase and cyclooxygenase inhibitors on carrageenan-induced pleurisy in rats.Life Sci,2006,79:442-447.
8. Di Paola R,Mazzon E,Muia C,et al.Green tea polyphenol extract attenuates lung injury in experimental model of carrageenan-induced pleurisy in mice.Respir Res,2005,6:66.
9. Agre P.The aquaporin water channels.Proc Am Thorac Soc,2006,3:5-13.
10. King LS,Nielsen S,Agre P.Aquaporins and the respiratory system:advice for a lung investigator.J Clin Invest,2000,105:15-16.
11. Bai C,F(xiàn)ukuda N,Song Y,et al.Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice.J Clin Invest,1999,103:555-561.
12. Antony VB.Immunological mechanisms in pleural disease.Eur Respir J,2003,21:539-44.
  1. 1. Light RW,LeeYCG..Textbook of Pleural Diseases.Firsted.Oxford University Press Inc,London,2003.xiii.
  2. 2. Manley GT,F(xiàn)ujimura M,Ma T,et al.Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke.Nat Med,2000,6:159-163.
  3. 3. Cuzzocrea S,Mazzon E,Calabro G,et al.Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan.Am J Respir Crit Care Med,2000,162:1859-1866.
  4. 4. 孫明瑜,謝鳴,張娜,等.小柴胡湯及其藥群配伍對角叉菜膠誘導(dǎo)大鼠胸膜炎模型細(xì)胞因子的影響.中國中西醫(yī)結(jié)合雜志,2004,24:628-631.
  5. 5. Corsini E,Di Paola R,Viviani B,Genovese T,Mazzon E,Lucchi L,Marinovich M,Galli CL,Cuzzocrea S.Increased carrageenan-induced acute lung inflammation in old rats.Immunology.2005 Jun;115:253-61.
  6. 6. Marzocco S,Di Paola R,Serraino I,et al.Effect of methylguanidine in carrageenan-induced acute inflammation in the rats.Eur J Pharmacol,2004,484:341-350.
  7. 7. Sakaguchi Y,Shirahase H,Kunishiro K,et al.Effect of combination of nitric oxide synthase and cyclooxygenase inhibitors on carrageenan-induced pleurisy in rats.Life Sci,2006,79:442-447.
  8. 8. Di Paola R,Mazzon E,Muia C,et al.Green tea polyphenol extract attenuates lung injury in experimental model of carrageenan-induced pleurisy in mice.Respir Res,2005,6:66.
  9. 9. Agre P.The aquaporin water channels.Proc Am Thorac Soc,2006,3:5-13.
  10. 10. King LS,Nielsen S,Agre P.Aquaporins and the respiratory system:advice for a lung investigator.J Clin Invest,2000,105:15-16.
  11. 11. Bai C,F(xiàn)ukuda N,Song Y,et al.Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice.J Clin Invest,1999,103:555-561.
  12. 12. Antony VB.Immunological mechanisms in pleural disease.Eur Respir J,2003,21:539-44.
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