• 1. 華西醫(yī)科大學附屬第一醫(yī)院普外科(成都610041)2. 華西醫(yī)科大學基礎(chǔ)醫(yī)學院生化教研室;

為研究肝細胞低密度脂蛋白受體對膽汁中膽汁酸的影響及其在膽囊結(jié)石過程中可能的機理，采用高膽固醇膳食誘發(fā)兔膽囊結(jié)石模型，對進食后1、2、3、4周組及對照組動物，采用雙波長薄層掃描法測定膽汁中膽汁酸，125I-LDL放免標記法測定肝細胞低密度脂蛋白受體(LDLr)活性。結(jié)果： 高膽固醇膳食后2、3、4周組分別有4/10，6/10，7/10出現(xiàn)膽囊結(jié)石； 膽汁中甘氨膽酸明顯降低(Ｐ＜0.05)；125I-LDL與肝細胞低密度脂蛋白受體(LDLr)的最大結(jié)合力(Bmax)逐漸下降(Ｐ＜0.05)，解離常數(shù)(Kd)值逐漸升高(Ｐ＜0.05)。上述結(jié)果提示： 肝細胞低密度脂蛋白受體活性下降，可能導(dǎo)致膽汁中甘氨膽酸減少，致成石性膽汁形成。

引用本文： 趙紀春,舒曄,侯彩芳,程南生,肖路加,朱紅. 肝細胞低密度脂蛋白受體活性對膽汁中膽汁酸影響的實驗研究. 中國普外基礎(chǔ)與臨床雜志, 1999, 6(4): 207-209. doi: 復(fù)制

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1. 趙紀春， 肖路加， 朱紅等. 兔膽囊結(jié)石形成過程中脂代謝和載脂蛋白變化研究. 華西醫(yī)科大學學報， 1997； 28(4)∶361.
2. 孟高淵， 東云華， 李彬等. 九種混合膽汁酸的薄層光密度定量分析. 華西預(yù)防醫(yī)學， 1989； 43(1)∶24.
3. Makwell MA. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Ann Biochem, 1978; 87(2)∶206.
4. Brown MS, Goldstein JL. A receptormediated pathway for cholesterol homeostasis. Science, 1986; 232(1)∶34.
5. Seglen PO. Preparation of rat liver cells. Exp Cell Res, 1993; 82(2)∶391.
6. Kern F Jr. Effects of dietary cholesterol on cholesterol and bile acid homeostasis in patients with cholesterol gallstones. J Clin Invest, 1994; 93(3)∶1186.
7. Rudel L, Deckelman C, Wilson M, et al. Dietary cholesterol and down regulation of cholesterol 7αhydroxylase and cholesterol absorption in African green monkeys. J Clin Invest, 1994; 93(8)∶2463.
8. Khallon J, Riottot M, Parquet M, et al. Biodynamics of cholesterol and bile acids in the lithasic hamster. Br J Nutr, 1991; 66(3)∶479.
9. Hayes KC, Livingston A, Trautwein EA. Dietary impact on biliary lipids and gallstones. Ann. Rev Nutr, 1992; 12∶299.
10. Nicolosi RJ, Stucchi AF, Kowala MC, et al. Effect of dietary fat saturation and cholesterol on LDL composition and metabolism. Arteriosclerosis, 1990; 10(1)∶119.
11. Everson GT, Daggy BP, Mckinley GT, et al. Dietary fiber (psyllium) lowers LDL cholesterol by stimulating bile acid synthesis in man. Hepatology, 1991; 14(Suppl)∶148a.
12. Reihner E, Angelin B, Rudling M, et al. Regulation of hepatic cholesterol metabolism in human: stimulatory effects of cholestyramine on HMGCoA reductase activity and low density lipoprotein receptor expression in gallstone patients. J Lipid Res, 1990; 31(12)∶2219.
13. Nanjee MN, Miller NE. Human hepatic low density lipoprotein receptors : associations of receptor activities in vitro with plasma lipid and apolipoprotein concentrations in vivo. Biochem Biophys Acta, 1989; 1002(2)∶245.
  1. 1. 趙紀春， 肖路加， 朱紅等. 兔膽囊結(jié)石形成過程中脂代謝和載脂蛋白變化研究. 華西醫(yī)科大學學報， 1997； 28(4)∶361.
  2. 2. 孟高淵， 東云華， 李彬等. 九種混合膽汁酸的薄層光密度定量分析. 華西預(yù)防醫(yī)學， 1989； 43(1)∶24.
  3. 3. Makwell MA. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Ann Biochem, 1978; 87(2)∶206.
  4. 4. Brown MS, Goldstein JL. A receptormediated pathway for cholesterol homeostasis. Science, 1986; 232(1)∶34.
  5. 5. Seglen PO. Preparation of rat liver cells. Exp Cell Res, 1993; 82(2)∶391.
  6. 6. Kern F Jr. Effects of dietary cholesterol on cholesterol and bile acid homeostasis in patients with cholesterol gallstones. J Clin Invest, 1994; 93(3)∶1186.
  7. 7. Rudel L, Deckelman C, Wilson M, et al. Dietary cholesterol and down regulation of cholesterol 7αhydroxylase and cholesterol absorption in African green monkeys. J Clin Invest, 1994; 93(8)∶2463.
  8. 8. Khallon J, Riottot M, Parquet M, et al. Biodynamics of cholesterol and bile acids in the lithasic hamster. Br J Nutr, 1991; 66(3)∶479.
  9. 9. Hayes KC, Livingston A, Trautwein EA. Dietary impact on biliary lipids and gallstones. Ann. Rev Nutr, 1992; 12∶299.
  10. 10. Nicolosi RJ, Stucchi AF, Kowala MC, et al. Effect of dietary fat saturation and cholesterol on LDL composition and metabolism. Arteriosclerosis, 1990; 10(1)∶119.
  11. 11. Everson GT, Daggy BP, Mckinley GT, et al. Dietary fiber (psyllium) lowers LDL cholesterol by stimulating bile acid synthesis in man. Hepatology, 1991; 14(Suppl)∶148a.
  12. 12. Reihner E, Angelin B, Rudling M, et al. Regulation of hepatic cholesterol metabolism in human: stimulatory effects of cholestyramine on HMGCoA reductase activity and low density lipoprotein receptor expression in gallstone patients. J Lipid Res, 1990; 31(12)∶2219.
  13. 13. Nanjee MN, Miller NE. Human hepatic low density lipoprotein receptors : associations of receptor activities in vitro with plasma lipid and apolipoprotein concentrations in vivo. Biochem Biophys Acta, 1989; 1002(2)∶245.