應(yīng)用ABC免疫組化及半巢式PCR法分別對64例大腸癌組織中bcl-2的蛋白表達(dá)及重排進(jìn)行了檢測。結(jié)果：bcl-2基因異常表達(dá)與重排在大腸癌早期即已出現(xiàn)，隨著病程演變，發(fā)生率顯著增加；bcl-2基因的重排在有淋巴結(jié)轉(zhuǎn)移者亦明顯多于無淋巴結(jié)轉(zhuǎn)移者。兩法聯(lián)合應(yīng)用相比較，以重排更能反映大腸癌分子特性。本實(shí)驗(yàn)結(jié)果提示：bcl-2基因參與大腸癌發(fā)生發(fā)展過程的調(diào)節(jié)，并在大腸癌細(xì)胞的增殖、進(jìn)展及轉(zhuǎn)移中起重要作用；研究大腸癌bcl-2基因情況可用來預(yù)測治療效果和預(yù)后，亦為大腸癌治療設(shè)計(jì)新的抗癌藥物提供了新的靶分子。
引用本文： 駱成玉,李世擁. 大腸癌bcl-2基因表達(dá)和重排的研究. 中國普外基礎(chǔ)與臨床雜志, 1999, 6(1): 4-6. doi: 復(fù)制
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- 3. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: laboratory manuals. 2th ed. New York: Cold Spring Harbor Laboratory Press, 1989∶463-493.
- 4. Yuan R, Dowling P, Zucca E, et al. Detection of bcl2/JH rearrangement in follicular and diffuse lymphoma: concordant results of peripheral blood and bone marrow analysis at diagnosis. Br J Cancer, 1993; 67(5)∶922.
- 5. Hsu B, Marin MC, Brisbay S, et al. Expression of bcl2 gene confers multidrug resistance to chemotherapyinduced cell death. Cancer Bull, 1994; 46(1)∶125.
- 6. Fisher TC, Milner AE, Gregory CD, et al. Bcl2 modulation of apoptosis induced by anticancer drugs: resistance to thymidylate stress is independent of classical resistance pathways. Cancer Res, 1993; 53(15)∶3321.