• 解放軍第306醫(yī)院肝膽外科（北京100101）;

目的  探索山莨菪堿對大鼠肝臟缺血再灌注損傷的保護作用。方法  選雄性Wistar大鼠160只，分為正常對照組、缺血再灌注組、生理鹽水組和山莨菪堿組，觀察了肝臟缺血60分鐘及再灌注1、3、6、12及24小時后血漿內(nèi)皮素-1（ET-1）、透明質(zhì)酸（HA）和谷丙轉(zhuǎn)氨酶（ALT）含量變化及肝組織病理學變化。結(jié)果  肝臟缺血再灌注后，血漿ET-1、HA和ALT含量均顯著增高，同時肝臟瘀血很明顯； 肝臟缺血再灌注前用山莨菪堿后，血漿HA和ALT含量明顯降低，同時肝組織瘀血減輕。結(jié)論  山莨菪堿可改善再灌注后的肝臟微循環(huán)障礙，對大鼠肝臟缺血再灌注損傷有保護作用。

引用本文： 董滿庫,崔彥,陳昌瑋,王平,肖廣生,周立艷,李曉鷗,吉敏,劉子沛. 山莨菪堿對大鼠肝臟缺血再灌注損傷保護作用的實驗研究. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(5): 295-297. doi: 復制

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2. Yanagisawa M, Kurihara H, Kimuras E, et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells 〔Ｊ〕. Nature, 1988; 332(6163)∶411.
3. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation 〔Ｊ〕. Surg Today, 1994; 24(8)∶719.
4. Zhang JX, Pegoli W, Clemens MG, et al. Endothelin1 induces direct constriction of hepatic sinusoids 〔Ｊ〕. Am J Physiol, 1994; 266(29)∶G624.
5. Mitsuoka H, Suzuki S, Sakaguchi T, et al. Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury 〔Ｊ〕. Transplantation, 1999; 67(4)∶514.
6. Wang Y, Lawson JA, Jaeschke H. Differential effect of 2aminoethylisothiourea, an inhibitor of the inducible nitric oxide synthase, on microvascular blood flow and organ injury in models of hepatic ischemiareperfusion and endotoxemia 〔Ｊ〕. Shock, 1998; 10(1)∶20.
7. Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury 〔Ｊ〕. Hepatogastroenterology, 1999; 46(12)∶1452.
8. Cursio R, Gugenheim J, PanaiaFerrari P, et al. Improvement of normothermic rat liver ischemia/reperfusion by muramyl dipeptide 〔Ｊ〕. J Surg Res, 1998; 80(2)∶339.
9. Soejima Y, Yanaga K, Nishizaki T, et al. Effect of specific neutrophil elastase inhibitor on ischemia/reperfusion injury in rat liver transplantation 〔Ｊ〕. J Surg Res, 1999; 86(1)∶150.
10. 邢卉春, 趙龍鳳, 王守義. 山莨菪堿對大鼠急性肝損傷的保護作用及其機制 〔Ｊ〕. 中國危重病急救醫(yī)學, 1998; 10(11)∶658.
11. Wang WC, Lee YN. Effects of anisodamine and atropine on the microvasculature of liver, skeletal muscle and footpad skin in anesthetized rats 〔Ｊ〕. Yao Hsueh Hsueh Pao, 1992; 27(5)∶385.
  1. 1. Nauta RJ, Tsimoyiannis E, Urib M, et al. Oxygenderived free radical in hepatic ischemia reperfusion injury in rat 〔Ｊ〕. Surg Gynecol Obstet, 1990; 171(2)∶120.
  2. 2. Yanagisawa M, Kurihara H, Kimuras E, et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells 〔Ｊ〕. Nature, 1988; 332(6163)∶411.
  3. 3. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation 〔Ｊ〕. Surg Today, 1994; 24(8)∶719.
  4. 4. Zhang JX, Pegoli W, Clemens MG, et al. Endothelin1 induces direct constriction of hepatic sinusoids 〔Ｊ〕. Am J Physiol, 1994; 266(29)∶G624.
  5. 5. Mitsuoka H, Suzuki S, Sakaguchi T, et al. Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury 〔Ｊ〕. Transplantation, 1999; 67(4)∶514.
  6. 6. Wang Y, Lawson JA, Jaeschke H. Differential effect of 2aminoethylisothiourea, an inhibitor of the inducible nitric oxide synthase, on microvascular blood flow and organ injury in models of hepatic ischemiareperfusion and endotoxemia 〔Ｊ〕. Shock, 1998; 10(1)∶20.
  7. 7. Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury 〔Ｊ〕. Hepatogastroenterology, 1999; 46(12)∶1452.
  8. 8. Cursio R, Gugenheim J, PanaiaFerrari P, et al. Improvement of normothermic rat liver ischemia/reperfusion by muramyl dipeptide 〔Ｊ〕. J Surg Res, 1998; 80(2)∶339.
  9. 9. Soejima Y, Yanaga K, Nishizaki T, et al. Effect of specific neutrophil elastase inhibitor on ischemia/reperfusion injury in rat liver transplantation 〔Ｊ〕. J Surg Res, 1999; 86(1)∶150.
  10. 10. 邢卉春, 趙龍鳳, 王守義. 山莨菪堿對大鼠急性肝損傷的保護作用及其機制 〔Ｊ〕. 中國危重病急救醫(yī)學, 1998; 10(11)∶658.
  11. 11. Wang WC, Lee YN. Effects of anisodamine and atropine on the microvasculature of liver, skeletal muscle and footpad skin in anesthetized rats 〔Ｊ〕. Yao Hsueh Hsueh Pao, 1992; 27(5)∶385.