• 四川大學(xué)華西醫(yī)院感染性疾病分子生物學(xué)重點(diǎn)實(shí)驗(yàn)室·四川大學(xué)教育部人類(lèi)疾病生物治療重點(diǎn)實(shí)驗(yàn)室(成都610041);;
  • 2.成都盛進(jìn)生物工程有限公司（成都610041）;;
  • 3.四川大學(xué)華西醫(yī)院普外科;

目的研究原發(fā)性肝細(xì)胞肝癌（HCC）中Rb基因的表達(dá)。方法利用組織芯片技術(shù)和免疫組織化學(xué)技術(shù)（SP法）檢測(cè)40例HCC及其相應(yīng)癌旁、遠(yuǎn)癌正常組織中Rb蛋白的表達(dá)情況。結(jié)果40例原發(fā)性HCC患者標(biāo)本中，腫瘤組織中Rb蛋白表達(dá)缺失者有17例，癌旁組織中有4例，在遠(yuǎn)癌正常組織有1例，HCC腫瘤組織中Rb蛋白缺失率顯著高于相應(yīng)癌旁及遠(yuǎn)癌正常組織（P＜0.05）； Rb蛋白表達(dá)的缺失與腫瘤的大小、病理學(xué)分級(jí)無(wú)統(tǒng)計(jì)學(xué)相關(guān)性(P gt;0.05)。結(jié)論HCC的發(fā)生、發(fā)展與Rb蛋白的缺失可能有關(guān)。組織芯片是一種可高效進(jìn)行腫瘤分子病理研究的技術(shù)平臺(tái)。

引用本文： 劉凱何煦趙連三周思亮劉麗劉聰劉自明嚴(yán)律南. 利用組織芯片研究Rb基因在原發(fā)性肝細(xì)胞肝癌中的表達(dá)△. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(3): 177-179. doi: 復(fù)制

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