目的 探討生長(zhǎng)抑素類似物SMS201995 (SMS) 對(duì)人膽管癌細(xì)胞體外增殖和凋亡的調(diào)控作用。方法采用人膽管癌細(xì)胞系SKChA1,應(yīng)用生長(zhǎng)曲線、流式細(xì)胞儀、片段DNA瓊脂糖凝膠電泳、Annexin VFITC技術(shù)及流式細(xì)胞儀間接免疫熒光技術(shù)等方法進(jìn)行檢測(cè)和觀察。結(jié)果在SMS 作用下，SKChA1細(xì)胞生長(zhǎng)受到抑制，短時(shí)使細(xì)胞阻滯于G0/G1期。SMS處理SKChA1細(xì)胞后, Annexin VFITC標(biāo)記的凋亡細(xì)胞增多, DNA凝膠電泳顯示典型的凋亡梯狀圖譜,同時(shí)SMS可使細(xì)胞bcl2蛋白表達(dá)下降,使bax蛋白表達(dá)升高。結(jié)論SMS 可明顯抑制SKChA1細(xì)胞生長(zhǎng)，并能誘導(dǎo)該細(xì)胞凋亡。該細(xì)胞凋亡機(jī)理可能與凋亡調(diào)控基因bax和bcl2的表達(dá)有關(guān)。
引用本文： 陳華,何振平,馬寬生,王曙光. 生長(zhǎng)抑素類似物對(duì)人膽管癌細(xì)胞增殖和凋亡的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(3): 164-167. doi: 復(fù)制
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- 2. Shih SC, Stutman O. Cell cycledependent tumor necrosis factor apoptosis [J]. Cancer Res,1996； 56(7)∶1591.
- 3. Teplan I. Peptides and antitumor activity. Development and investigation of some peptides with antitumor activity [J]. Acta Biol Hung， 2000； 51(1)∶1.
- 4. Dancygier H， Klein U， Leuschner U， et al. Somatostatincontaining cells in the extrahepatic biliary tract of humans [J]. Gastroenterology，1984； 86(5 Pt 1)∶892.
- 5. Pagliacci MC. Inhibition of human breast cancer cell (MCF7) growth in vitro by the somatostain analog SMS201995: effects on cell cycle parameters and apoptotic cell death [J]. Endocrinology, 1991； 129(5)∶2555.
- 6. Cheung NW, Boyages SC. Somatostatin14 and its analog octreotide exert a cytostatic effect on GH3 rat pituitary tumor cell proliferation via a transient G0/G1 cell cycle block [J].Endocrinology,1995； 136(10)∶4174.
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- 8. Brinder C, Marx D, Binder L, et al. Expression of bax in relation to bcl2 and other predictive parameters in breast cancer [J].Ann Oncol,1996； 7(2)∶129.