• 1.四川大學(xué)華西醫(yī)院普外科 (成都610041);;
  • 2.四川大學(xué)華西基礎(chǔ)醫(yī)學(xué)與法醫(yī)學(xué)院生化教研室;;
  • 3.四川大學(xué)華西基礎(chǔ)醫(yī)學(xué)與法醫(yī)學(xué)院檢測(cè)中心;;
  • 4.四川大學(xué)華西公共衛(wèi)生學(xué)院病理科;

目的 探討肝細(xì)胞膜鈉泵(Na+K+ATPase)活性變化在膽紅素鈣結(jié)石成石過程中的作用。方法采用膽紅素鈣結(jié)石兔模型進(jìn)行研究，對(duì)照組28只，膽道不全梗阻組（BO組）36只，膽道不全梗阻加感染組（BOI組）39只，各組再按術(shù)后處死時(shí)間3 d、7 d、14 d及20 d時(shí)相分組。動(dòng)態(tài)測(cè)定各組各時(shí)相肝細(xì)胞膜鈉泵活性、肝細(xì)胞鈣及膽汁中膽汁酸含量。 結(jié)果BO組及BOI組肝細(xì)胞膜鈉泵活性及膽汁中膽汁酸含量進(jìn)行性下降，肝細(xì)胞鈣含量呈進(jìn)行性增多，與對(duì)照組相比差異有顯著性意義（P＜0.01），但BOI組上述變化較BO組明顯（P＜0.05）。結(jié)論膽紅素鈣結(jié)石成石過程中存在肝細(xì)胞膜鈉泵活性的進(jìn)行性下降，鈉泵活性下降引起肝細(xì)胞鈣超負(fù)荷及膽汁中膽汁酸含量下降，從而促進(jìn)成石。細(xì)菌感染加重上述變化，使結(jié)石形成增多。

引用本文： 莊文,肖路加,左鳳瓊,東云華,吳忠舜,吳兆鋒. 肝細(xì)胞膜鈉泵活性變化在膽紅素鈣結(jié)石成石過程中的作用. 中國普外基礎(chǔ)與臨床雜志, 2002, 9(2): 73-75. doi: 復(fù)制

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4. Jame L, Merion RM, Burtch GD, et al. Properties of Na+K+activated ATPase in rat liver plasma membranes enriched with bile canaliculi [J]. Biochim Biophys Acta, 1975; 401(3)∶59.
5. Schanne FA, Muller C, Endlich K, et al. Liver plasma membrane calcium transport [J]. J Biol Chem, 1986; 261(21)∶9886.
6. Michael H, Garrel T, Decurtins M, et al. Mechanisms and regulations of bile secretion [J]. Hepatology, 1991; 14(3)∶551.
7. Frimmer M, Grant B, Zobeck TS, et al. The transport of bile acids in liver cells [J]. Biochim Biophys Acta, 1988; 947(8)∶75.
8. Chung TY. Calcium and ischemic injury [J]. New Engl J Med, 1986;314(26)∶1670.
9. Victor A, Inan M, Sayek I, et al. Conditions that results in the mobilization and influx of Ca2+ into rat hepatocytes induce the rapid loss of HMGCoA reductase activity that is not reversed by phosphatase treatment [J]. Biochem J, 1990; 269(9)∶373.
  1. 1. 周孝思. 我國膽色素結(jié)石成因的研究狀況 [J]. 普外臨床，1994; 9(4)∶193.
  2. 2. 曾勇，肖路加，張尚福. 兔膽紅素鈣結(jié)石成石過程中膽囊免疫球蛋白分泌細(xì)胞含量的變化 [J]. 普外基礎(chǔ)與臨床雜志，1997; 4(2)∶75.
  3. 3. Meeda T, Bismuth H, Sherlock DJ, et al. A simple and rapid method for the preparation of plasma membranes [J]. Biochim Biophys Acta, 1983; 731(5)∶115.
  4. 4. Jame L, Merion RM, Burtch GD, et al. Properties of Na+K+activated ATPase in rat liver plasma membranes enriched with bile canaliculi [J]. Biochim Biophys Acta, 1975; 401(3)∶59.
  5. 5. Schanne FA, Muller C, Endlich K, et al. Liver plasma membrane calcium transport [J]. J Biol Chem, 1986; 261(21)∶9886.
  6. 6. Michael H, Garrel T, Decurtins M, et al. Mechanisms and regulations of bile secretion [J]. Hepatology, 1991; 14(3)∶551.
  7. 7. Frimmer M, Grant B, Zobeck TS, et al. The transport of bile acids in liver cells [J]. Biochim Biophys Acta, 1988; 947(8)∶75.
  8. 8. Chung TY. Calcium and ischemic injury [J]. New Engl J Med, 1986;314(26)∶1670.
  9. 9. Victor A, Inan M, Sayek I, et al. Conditions that results in the mobilization and influx of Ca2+ into rat hepatocytes induce the rapid loss of HMGCoA reductase activity that is not reversed by phosphatase treatment [J]. Biochem J, 1990; 269(9)∶373.