• 1.上海第二醫(yī)科大學(xué)附屬瑞金醫(yī)院外科（上海200025）;;
  • 2.江西醫(yī)學(xué)院第二附屬醫(yī)院普外科（南昌330006）;

淋巴結(jié)轉(zhuǎn)移是胃癌行根治性切除術(shù)后轉(zhuǎn)移和復(fù)發(fā)的主要途徑之一，有無淋巴結(jié)轉(zhuǎn)移及轉(zhuǎn)移淋巴結(jié)數(shù)目的多少與胃癌的預(yù)后密切相關(guān)［1］。常規(guī)組織學(xué)檢查淋巴結(jié)有可能忽略微轉(zhuǎn)移的存在，這將直接影響著臨床分期的準確性、預(yù)后預(yù)測以及輔助治療的選擇。為此，我們采用靶向癌胚抗原（CEA）mRNA的巢式逆轉(zhuǎn)錄聚合酶鏈反應(yīng)（Nested RTPCR）方法，檢測常規(guī)病理檢查淋巴結(jié)陰性胃癌的淋巴結(jié)微轉(zhuǎn)移情況并探討其臨床意義。

引用本文： 肖衛(wèi)東,彭承宏,王夢龍,鄒葉青,程華,朱培謙. RT-PCR法檢測常規(guī)病理檢查淋巴結(jié)陰性胃癌的淋巴結(jié)微轉(zhuǎn)移. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(5): 487-488. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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  2. 2. Mori M, Mimori K, Inoue H, et al. Detection of cancer micrometastasis in lymph nodes by reverse transcriptasepolymerase chain reaction [Ｊ]. Cancer Res, 1995; 55(15)∶3417.
  3. 3. Brown DC, Purushotham AD, Birnie GD, et al. Detection of intraoperative tumor cell dissemination in patients with breast cancer by use of reverse transcription and polymerase chain reaction [Ｊ]. Surgery, 1995; 117(1)∶95.
  4. 4. Japanses Gastric Carcinoma Association. Japanese Classification of gastric carcinoma2nd English Edition [Ｊ]. Gastric Cancer, 1998; 1(1)∶10.
  5. 5. Roder JD, Bottcher K, Busch R, et al. Classification of regional lymph node metastasis from gastric carcinoma [Ｊ]. Cancer, 1998; 82(4)∶621.
  6. 6. Baba H, Maehara Y, Takeuchi H, et al. Effect of lymph node dissection on the prognosis in patients with nodenegative early gastric cancer [Ｊ]. Surgery, 1995; 117(2)∶165.
  7. 7. Mori M, Mimori K, Ueo H, et al. Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcriptionpolymerase chain reaction in patients with gastrointestinal or breast carcinomas [Ｊ]. J Clin Oncol, 1998; 16(1)∶128.
  8. 8. Harrison LE, Choe JK, Goldstein M, et al. Prognostic significance of immunohistochemical micrometastases in node negative gastric cancer patients [Ｊ]. J Surg Oncol, 2000; 73(3)∶153.
  9. 9. Nakajo A, Natsugoe S, Ishigamis S, et al. Detection and prodiction of micrometastasis in the lymph nodes of patients with pN0 gastric cancer [Ｊ]. Ann Surg Oncol, 2001; 8(2)∶158.