目的探討鼠肝大部切除術(shù)后空、回腸粘膜固有層細(xì)胞免疫功能的變化及其與腸道細(xì)菌移位的關(guān)系。方法將48只SD大鼠隨機(jī)分為實(shí)驗(yàn)組和假手術(shù)組,每組24只。實(shí)驗(yàn)組切除70%肝臟,假手術(shù)組除不切除肝臟外,其余手術(shù)步驟同實(shí)驗(yàn)組。分別于術(shù)后6、12、24和72 h取兩組大鼠(n=6)空、回腸粘膜冰凍切片,而后行免疫組化染色,觀察不同時(shí)相腸粘膜固有層CD3+、CD4+、CD8+ T淋巴細(xì)胞的數(shù)量及肝臟功能的變化。結(jié)果實(shí)驗(yàn)組術(shù)后24 h和72 h,其腸粘膜固有層CD3+、CD4+和CD8+T淋巴細(xì)胞的數(shù)量較假手術(shù)組明顯下降(P<0.05),而兩組大鼠術(shù)后不同時(shí)相的ALT及AST變化,實(shí)驗(yàn)組明顯高于假手術(shù)組(P lt;0.05)。結(jié)論鼠肝大部切除(70%)術(shù)后24 h,腸粘膜固有層CD3+、CD4+和CD8+ T淋巴細(xì)胞數(shù)量明顯降低。這種細(xì)胞免疫功能降低所導(dǎo)致的腸粘膜屏障功能受損,可能是造成腸道細(xì)菌移位的原因之一。
引用本文: 楊之斌,張炳彥,朱洪,王炳煌,郭永章. 鼠肝大部切除術(shù)后腸粘膜細(xì)胞免疫功能的變化及其與腸道細(xì)菌移位的關(guān)系. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(5): 469-470. doi: 復(fù)制
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