• 廣西醫(yī)科大學第一附屬醫(yī)院外科(南寧530021);

目的用腫瘤壞死因子α(TNFα)基因轉(zhuǎn)導的人肝癌細胞作為疫苗接種小鼠,研究其對小鼠肝癌移植瘤的免疫排斥作用。方法取昆明種小鼠40只,分為5組,每組8只。分別采用經(jīng)60Co照射的TNFα基因轉(zhuǎn)導的人肝癌細胞(BEL7404TNFCo組)、未經(jīng)60Co照射的TNFα基因轉(zhuǎn)導的人肝癌細胞(BEL7404TNF組)、經(jīng)60Co照射的人肝癌細胞(BEL7404Co組)以及未經(jīng)60Co照射的人肝癌細胞(BEL7404組)接種小鼠,生理鹽水對照組僅注射生理鹽水。然后將小鼠肝癌細胞株H22移植于上述5組小鼠,觀察肝癌移植瘤生長情況,并應用脫氧核糖核酸末端轉(zhuǎn)移酶介導的缺口末端標記(TUNEL)法觀察腫瘤細胞凋亡指數(shù)。結(jié)果BEL7404TNFCo組小鼠接種經(jīng)60Co照射的TNFα基因轉(zhuǎn)導的人肝癌細胞疫苗后,肝癌移植瘤成瘤率下降,瘤體生長受抑制,細胞凋亡指數(shù)增高,與BEL7404Co組、BEL7404組及生理鹽水對照組相比,差異有顯著性(P<0.01),但與BEL7404TNF組相比,差異則無統(tǒng)計學意義(P gt;0.05)。結(jié)論TNFα基因轉(zhuǎn)導的人肝癌細胞疫苗對小鼠肝癌移植瘤有較強的免疫排斥作
用。

引用本文: 陸云飛,覃新干,曾健,林進令,廖清華. 腫瘤壞死因子α基因轉(zhuǎn)導的人肝癌細胞疫苗對小鼠肝癌移植瘤的影響. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(4): 366-369. doi: 復制

版權(quán)信息: ?四川大學華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有,未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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6. Koshita Y, Lu Y, Fujii S, et al. Efficacy of TNFalpha genetransduced tumor cells in treatment of established in vivo tumor [J]. Int J Cancer, 1995; 63(1)∶130.
7. 陸云飛, 陽杰武, 羅意革, 等. 外源性TNF基因?qū)θ烁伟┘毎L的影響 [J]. 中國腫瘤生物治療雜志, 1999; 6(3)∶193.
  1. 1. Finn OJ. Tumorspecific immune responses and opportunities for tumor vaccines [J]. Clin Immunol Immunopathol, 1994; 71(3)∶260.
  2. 2. 陸云飛,林進令,邱慶明,等. 原發(fā)性肝癌基因治療研究含人TNF基因重組逆轉(zhuǎn)錄病毒轉(zhuǎn)移系統(tǒng)的構(gòu)建與表達 [J]. 廣西醫(yī)科大學學報, 1997; 14(4)∶1.
  3. 3. 邱慶明,陸云飛. 轉(zhuǎn)基因人肝癌細胞株(BEL7404/TNF)的建立及其生物活性的初步研究 [J]. 廣西醫(yī)科大學學報, 1999; 16(4)∶408.
  4. 4. Wright P, Braun R, Babiuk L, et al. Adenovirusmediated TNFalpha gene transfer induces significant tumor regression in mice [J]. Cancer Biother Radiopharm, 1999; 14(1)∶49.
  5. 5. Sato Y, Koshita Y, Hirayama M, et al. Augmented antitumor effects of killer cells induced by tumor necrosis factor genetransdued autologous tumor cells from gastrointestinal cancer patients [J]. Hum Gene Ther, 1996; 7(15)∶1895.
  6. 6. Koshita Y, Lu Y, Fujii S, et al. Efficacy of TNFalpha genetransduced tumor cells in treatment of established in vivo tumor [J]. Int J Cancer, 1995; 63(1)∶130.
  7. 7. 陸云飛, 陽杰武, 羅意革, 等. 外源性TNF基因?qū)θ烁伟┘毎L的影響 [J]. 中國腫瘤生物治療雜志, 1999; 6(3)∶193.