目的探討T細(xì)胞疫苗誘導(dǎo)特異性免疫耐受與外周血T細(xì)胞凋亡的關(guān)系。方法制備針對Wistar大鼠的SD大鼠T細(xì)胞疫苗，然后用該疫苗免疫健康SD大鼠，每周1次, 連續(xù)3周，作為實(shí)驗(yàn)組（n=6）。對照組（n=6）用RPMI 1640培養(yǎng)液替代T細(xì)胞疫苗。以被免疫的SD大鼠的脾細(xì)胞作為反應(yīng)細(xì)胞，以Wistar大鼠的脾細(xì)胞作為刺激細(xì)胞(經(jīng)絲裂霉素處理)，于接種前和每次接種后第5天進(jìn)行單向混合淋巴細(xì)胞反應(yīng)（3HTDR摻入法）,測定其cpm值； 用流式細(xì)胞儀對外周血T細(xì)胞凋亡情況進(jìn)行檢測。結(jié)果混合淋巴細(xì)胞反應(yīng)結(jié)果顯示，實(shí)驗(yàn)組SD大鼠脾細(xì)胞免疫應(yīng)答能力于接種后受到顯著抑制，其cpm值較接種前顯著降低 (P＜0.01)； 對照組接種前后各時點(diǎn)比較，其差異無顯著性（P gt;0.05）； 接種后相同時點(diǎn)的cpm值組間比較, 實(shí)驗(yàn)組顯著低于對照組(P＜0.01)； 與接種前比較，接種后實(shí)驗(yàn)組外周血T細(xì)胞凋亡率顯著增高(P lt;0.01)，且隨著疫苗接種次數(shù)的增加而升高，而對照組接種后各時點(diǎn)T細(xì)胞凋亡率與接種前比較差異無顯著性（P gt;0.05）。結(jié)論T細(xì)胞疫苗可以誘導(dǎo)同種特異性免疫耐受，其機(jī)理可能是通過誘導(dǎo)外周血T細(xì)胞凋亡，清除抗原特異性反應(yīng)性T細(xì)胞克隆來實(shí)現(xiàn)的。
引用本文： 趙振林,郭永章,李立,張捷,張瑞. T細(xì)胞疫苗誘導(dǎo)免疫耐受與外周血T細(xì)胞凋亡的關(guān)系探討. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(4): 359-361. doi: 復(fù)制
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