目的探討T細(xì)胞疫苗誘導(dǎo)特異性免疫耐受與外周血T細(xì)胞凋亡的關(guān)系。方法制備針對Wistar大鼠的SD大鼠T細(xì)胞疫苗,然后用該疫苗免疫健康SD大鼠,每周1次, 連續(xù)3周,作為實驗組(n=6)。對照組(n=6)用RPMI 1640培養(yǎng)液替代T細(xì)胞疫苗。以被免疫的SD大鼠的脾細(xì)胞作為反應(yīng)細(xì)胞,以Wistar大鼠的脾細(xì)胞作為刺激細(xì)胞(經(jīng)絲裂霉素處理),于接種前和每次接種后第5天進(jìn)行單向混合淋巴細(xì)胞反應(yīng)(3HTDR摻入法),測定其cpm值; 用流式細(xì)胞儀對外周血T細(xì)胞凋亡情況進(jìn)行檢測。結(jié)果混合淋巴細(xì)胞反應(yīng)結(jié)果顯示,實驗組SD大鼠脾細(xì)胞免疫應(yīng)答能力于接種后受到顯著抑制,其cpm值較接種前顯著降低 (P<0.01); 對照組接種前后各時點(diǎn)比較,其差異無顯著性(P gt;0.05); 接種后相同時點(diǎn)的cpm值組間比較, 實驗組顯著低于對照組(P<0.01); 與接種前比較,接種后實驗組外周血T細(xì)胞凋亡率顯著增高(P lt;0.01),且隨著疫苗接種次數(shù)的增加而升高,而對照組接種后各時點(diǎn)T細(xì)胞凋亡率與接種前比較差異無顯著性(P gt;0.05)。結(jié)論T細(xì)胞疫苗可以誘導(dǎo)同種特異性免疫耐受,其機(jī)理可能是通過誘導(dǎo)外周血T細(xì)胞凋亡,清除抗原特異性反應(yīng)性T細(xì)胞克隆來實現(xiàn)的。
引用本文: 趙振林,郭永章,李立,張捷,張瑞. T細(xì)胞疫苗誘導(dǎo)免疫耐受與外周血T細(xì)胞凋亡的關(guān)系探討. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(4): 359-361. doi: 復(fù)制
版權(quán)信息: ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有,未經(jīng)授權(quán)不得轉(zhuǎn)載、改編
1. | Shapira OM, Mor E, Reshef T, et al. Prolongation of survival of rat cardiac allografts by T cell vaccination [J]. J Clin Invest, 1993; 91(2)∶388. |
2. | Gudmundsdottir H, Turka LA. Transplantation tolerance: mechanisms and strategies? [J]. Semin Nephrol, 2000; 20(2)∶209. |
3. | 李天發(fā),屠宏慶.移植免疫耐受、移植排斥與細(xì)胞凋亡 [J]. 國外醫(yī)學(xué)免疫性分冊, 2000; 23(3)∶162. |
4. | Hermans G, Denzer U, Lohse A,et al. Cellular and humoral immune responses against autoreactive T cells in multiple sclerosis patients after T cell vaccination [J]. J Autoimmun, 1999; 13(2)∶233. |
5. | Zang YC, Hong J, Rivera VM, et al. Preferential recognition of TCR hypervarible regions by human antiidiotypic T cells induced by T cell vaccination [J]. J Immunol, 2000; 164(8)∶4011. |
6. | 陳永良,何球藻. 移植免疫中T細(xì)胞疫苗作用機(jī)制的初步分析 [J]. 上海免疫學(xué)雜志, 1996; 16(3)∶138. |
7. | Kabelitz D, Pohl T,Pechhold K.T cell apoptosis triggered via the CD3/T cell receptor complex and alternative activation pathways [J]. Curr Top Microbiol Immunol, 1995; 200(1)∶1. |
- 1. Shapira OM, Mor E, Reshef T, et al. Prolongation of survival of rat cardiac allografts by T cell vaccination [J]. J Clin Invest, 1993; 91(2)∶388.
- 2. Gudmundsdottir H, Turka LA. Transplantation tolerance: mechanisms and strategies? [J]. Semin Nephrol, 2000; 20(2)∶209.
- 3. 李天發(fā),屠宏慶.移植免疫耐受、移植排斥與細(xì)胞凋亡 [J]. 國外醫(yī)學(xué)免疫性分冊, 2000; 23(3)∶162.
- 4. Hermans G, Denzer U, Lohse A,et al. Cellular and humoral immune responses against autoreactive T cells in multiple sclerosis patients after T cell vaccination [J]. J Autoimmun, 1999; 13(2)∶233.
- 5. Zang YC, Hong J, Rivera VM, et al. Preferential recognition of TCR hypervarible regions by human antiidiotypic T cells induced by T cell vaccination [J]. J Immunol, 2000; 164(8)∶4011.
- 6. 陳永良,何球藻. 移植免疫中T細(xì)胞疫苗作用機(jī)制的初步分析 [J]. 上海免疫學(xué)雜志, 1996; 16(3)∶138.
- 7. Kabelitz D, Pohl T,Pechhold K.T cell apoptosis triggered via the CD3/T cell receptor complex and alternative activation pathways [J]. Curr Top Microbiol Immunol, 1995; 200(1)∶1.