• 中國醫(yī)學(xué)科學(xué)院中國協(xié)和醫(yī)科大學(xué)北京協(xié)和醫(yī)院外科(北京100730);

目的通過檢測腫瘤壞死因子相關(guān)細胞凋亡誘導(dǎo)配體受體-4（TRAIL-R4）在胰腺癌組織中的表達，探討胰腺癌細胞抵抗細胞凋亡的機理。方法應(yīng)用mRNA印跡法（Northern blotting）、蛋白質(zhì)印跡法（Western blotting）和免疫組織化學(xué)技術(shù)，定性、定位分析TRAIL-R4在正常胰腺組織和胰腺癌組織中的表達。結(jié)果TRAIL-R4 mRNA和蛋白在正常胰腺組織中呈弱表達或不表達，而在胰腺癌組織中呈高表達； 免疫組織化學(xué)檢測顯示，在胰腺癌細胞中TRAIL-R4蛋白呈強染色。結(jié)論TRAIL-R4表達水平在正常胰腺組織與胰腺癌組織中存在顯著差異，提示胰腺癌細胞中可能存在對TRAIL介導(dǎo)的細胞凋亡抵抗新機理。

引用本文： 廖泉,郭俊超,趙玉沛. 腫瘤壞死因子相關(guān)細胞凋亡誘導(dǎo)配體受體-4在人胰腺癌組織中的表達. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(6): 535-538. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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