目的 研究大鼠甲狀旁腺細胞經(jīng)培養(yǎng)后再移植對其存活的影響。
方法 將經(jīng)膠原酶和胰蛋白酶消化的甲狀旁腺細胞培養(yǎng)后再行移植，觀察移植物的存活情況，并對移植物做透射電鏡觀察。
結果 新鮮甲狀旁腺移植組平均存活期為(9.25±3.45) d； 甲狀旁腺細胞培養(yǎng)后移植，移植物的存活時間為(46.25±7.44) d，明顯延長 (P＜0.01)，在50 d觀察期內(nèi)，8只鼠中有6只的血清鈣及PTH值持續(xù)在正常范圍內(nèi)。移植物內(nèi)可見完整的甲狀旁腺細胞，其內(nèi)見豐富的粗面內(nèi)質(zhì)網(wǎng)、桿狀的線粒體及分泌顆粒。
結論 大鼠甲狀旁腺細胞經(jīng)培養(yǎng)后再移植可以延長移植物的存活時間，是治療甲狀旁腺功能低下癥的一條有效途徑。
引用本文： 段秀慶,宋純,王江濱,石于波,許評,宋春芳. 大鼠甲狀旁腺細胞經(jīng)培養(yǎng)后再移植的實驗研究. 中國普外基礎與臨床雜志, 2004, 11(6): 486-488. doi: 復制
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- 2. Starling JR, Fidler R, Corry RJ. Prolongation of survival of rat parathyroid allografts by enhancing serum and tissue culture [Ｊ].Surgery, 1977; 81(6)∶668.
- 3. Feind CR, Weber CJ, Derenoncourt F, et al. Survival and allotransplantation of cultured human parathyroids [Ｊ].Transplant Proc, 1979; 11(1)∶1011.
- 4. Schachter P, Christy MD, Leight GS Jr, et al. Function in athymic nude mice of parathyroid heterografts from patients with primary hyperparathyroidism and secondary hyperparathyroidism [Ｊ].Surgery, 1990; 108(6)∶1040.
- 5. Tsuji K, Fuchinoue S, Kai K, et al. Culture of human parathyroid cells for transplantation [Ｊ].Transplant Proc, 1999; 31(7)∶2697.
- 6. Faustman D, Coe C. Prevention of xenograft rejection by masking donor HLA class I antigens [Ｊ]. Science, 1991; 252(5013)∶1700.
- 7. 王顏剛, 王桂蘭. 甲狀旁腺移植的進展 [Ｊ]. 國外醫(yī)學外科學分冊, 1994; 21(3)∶143.