目的探討移植靜脈中細(xì)胞凋亡及相關(guān)基因表達(dá)的動(dòng)態(tài)變化及意義。方法建立100只Wistar大鼠自體頸靜脈移植于腎下腹主動(dòng)脈模型。采用原位DNA片斷末端標(biāo)記(TUNEL)和免疫組織化學(xué)法,檢測(cè)移植靜脈血管平滑肌細(xì)胞(VSMCs)凋亡及相關(guān)基因bcl2和bax的表達(dá)。結(jié)果術(shù)后1~8周,移植靜脈凋亡的VSMCs多于對(duì)照靜脈(P<0.01); 術(shù)后2周為凋亡高峰,其凋亡細(xì)胞達(dá)(28.5±16.6)%,4~8周逐漸下降至(8.1±2.8)%;與對(duì)照靜脈〔(0.5±0.2)%〕相比差異有顯著性意義(P<0.01)。 術(shù)后1~2周,bcl2表達(dá)增多〔(22.1±5.4)%〕,與對(duì)照組〔(4.3±2.1)%〕及4~8周組間比較差異有顯著性意義(P<0.01); 移植后1~6周,bax表達(dá)顯著高于對(duì)照組〔(5.5±2.3)%〕及術(shù)后8周組〔(8.2±2.9)%〕,P<0.01。結(jié)論 bcl2與bax基因與移植靜脈VSMCs的凋亡密切相關(guān); VSMCs凋亡是移植靜脈重塑和狹窄的重要因素。
引用本文: 馮勇,胡海地,段志泉,呂慶杰. 凋亡相關(guān)基因bcl-2和bax在大鼠自體移植靜脈中的動(dòng)態(tài)表達(dá)及意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2004, 11(4): 295-299. doi: 復(fù)制
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- 1. 馮勇,胡海地,段志泉,等. 自體移植靜脈平滑肌細(xì)胞凋亡與增殖關(guān)系的實(shí)驗(yàn)研究 [J]. 中國(guó)醫(yī)科大學(xué)學(xué)報(bào), 2000; 29(2)∶103.
- 2. Reed JC. Bcl2 and the regulation of programmed cell death [J]. J Cell Biol, 1994; 124(1-2)∶1.
- 3. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death [J]. Cell, 1993; 74(4)∶609.
- 4. Gavrieli Y, Sherman Y, BenSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [J]. J Cell Biol, 1992; 119(3)∶493.
- 5. Bennett MR, Evan GI, Schwartz SM. Apoptosis of human vascular smooth muscle cells derived from normal vessels and coronary atherosclerotic plaques [J]. J Clin Invest, 1995; 95(5)∶2266.
- 6. 馮勇,胡海地,段志泉,等.大鼠自體移植靜脈術(shù)后血漿內(nèi)皮素、降鈣素基因相關(guān)肽與平滑肌細(xì)胞增殖的關(guān)系 [J]. 中華外科雜志, 2000; 5(38)∶400.
- 7. Krajewski S, Tanaka S, Takayama S, et al. Investigation of the subcellular distribution of the bcl2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes [J]. Cancer Res, 1993; 53(19)∶4701.
- 8. 徐宏勇,李開(kāi)宗,付由池,等. 雌激素受體和凋亡相關(guān)基因bcl及bax在人原發(fā)性膽囊癌中表達(dá)的臨床意義 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002; 9(2)∶86.