• 華中科技大學(xué)同濟醫(yī)學(xué)院附屬同濟醫(yī)院普外科(武漢430030);

目的  觀察膽囊結(jié)石患者膽汁(CB)對人膽囊癌細胞系GBC-SD生長的影響,探討膽囊結(jié)石與膽囊癌發(fā)生與發(fā)展的關(guān)系。
方法  應(yīng)用四唑藍(MTT)比色法檢測20例CB和20例正常膽汁(NB)對GBC-SD細胞增殖的影響,應(yīng)用流式細胞儀測定細胞周期和凋亡。
結(jié)果  CB與NB比較,前者可明顯促進GBC-SD細胞增殖,用CB處理48 h后GBC-SD細胞增殖指數(shù)顯著上升(P<0.05)。CB組S期細胞比例為(49.26±8.07)%,明顯高于NB組的(25.54±6.57)%,P<0.05; CB組G0/G1期細胞比例為(40.59±9.12)%,明顯低于NB組的(60.64±13.42)%,P<0.05。CB組和NB組細胞凋亡率分別為(5.50±1.16)%和(3.91±1.76)%,P gt;0.05。
結(jié)論  CB具有潛在的促增殖活性,且膽囊結(jié)石與膽囊癌的發(fā)生與發(fā)展關(guān)系密切。

引用本文: 吳高松,鄒聲泉. 膽囊結(jié)石患者膽汁對人膽囊癌細胞增殖的影響. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(2): 159-161. doi: 復(fù)制

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  2. 2. 石景森.膽囊癌與膽囊結(jié)石的相關(guān)研究 [J].中華肝膽外科雜志, 2001; 7(2)∶116.
  3. 3. 石景森,楊毅軍. 原發(fā)性膽囊癌與膽囊結(jié)石關(guān)系研究的新進展 [J].中華普通外科雜志, 2000; 15(6)∶373 .
  4. 4. 馬浙夫,王忠裕,張俊然.成石膽汁在膽囊癌致病因素中的作用 [J].中華醫(yī)學(xué)雜志, 2001; 81(5)∶268.
  5. 5. Wu GS, Zou SQ, Luo XW, et al. Proliferative activity of bile from congenital choledochal cyst patients [J]. World J Gastroenterol, 2003; 9(1)∶184.
  6. 6. Wu GS, Zou SQ, Liu ZR, et al. Bile from a patient with anomalous pancreaticobiliary ductal union promotes the proliferation of human cholangiocarcinoma cells via COX2 pathway [J].World J Gastroenterol, 2003; 9(5)∶1094.
  7. 7. Sugiyama Y, Kobori H, Hakamada K, et al. Altered bile composition in the gallbladder and common bile duct of patients with anomalous pancreaticobiliary ductal junction [J].World J Surg, 2000; 24(1)∶17.
  8. 8. Chijiiwa K, Nagai E, Makino I, et al. Are secondary bile acids in choledochal cysts important as a risk factor in biliary tract carcinoma? [J]. Aust N Z J Surg, 1993; 63(2)∶109.