• 1.云南省第一人民醫(yī)院腫瘤科（昆明650032）2.云南省第一人民醫(yī)院基礎(chǔ)醫(yī)學(xué)研究室;

目的 探討大腸癌患者外周血CK20 mRNA的表達(dá)及其意義。
方法 用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)（RT-PCR）方法檢測42例大腸癌患者術(shù)前、術(shù)后外周血及20例大腸癌患者新鮮癌組織標(biāo)本中CK20 mRNA的表達(dá)，另以20例健康成年自愿者作對照。
結(jié)果 42例大腸癌患者外周血CK20 mRNA呈陽性表達(dá)者術(shù)前為19例（45.24%），術(shù)后為14例（33.33%）； 20例大腸癌患者新鮮癌組織中CK20 mRNA表達(dá)均為陽性。而正常對照組20例外周血CK20 mRNA表達(dá)均為陰性。且大腸癌外周血CK20 mRNA陽性表達(dá)與組織學(xué)類型無關(guān)（P gt;0.05），但與有無淋巴結(jié)轉(zhuǎn)移、肝轉(zhuǎn)移及Dukes分期有關(guān)（P＜0.05）。
結(jié)論 檢測外周血中CK20 mRNA表達(dá)將有助于大腸癌的早期診斷、預(yù)后的判斷及治療方案的制定。

引用本文： 孫建偉,華映坤,賈玲,林人龍,楊昆憲,陳居敏. 大腸癌患者外周血CK20 mRNA的表達(dá)及其意義. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(2): 119-121. doi: 復(fù)制

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  2. 2. Bustin SA, Gyselman VG, Williams NS, et al. Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients [Ｊ]. Br J Cancer, 1999; 79(11-12)∶1813.
  3. 3. Peck K, Sher YP, Shih JY, et al. Detection and quantitation of circulating cancer cells in the peripheral blood of lung cancer patients [Ｊ]. Cancer Res, 1998; 58(13)∶2761.
  4. 4. Aihara T, Noguchi S, Ishikawa O, et al. Detection of pancreatic and gastric cancer cells in peripheral and portal blood by amplification of keratin 19 mRNA with reverse transcriptasepolymerase chain reaction [Ｊ]. Int J Cancer, 1997; 72(3)∶408.
  5. 5. Datta YH, Adams PT, Drobyski WR, et al. Sensitive detection of occult breast cancer by the reversetranscriptase polymerase chain reaction [Ｊ]. J Clin Oncol, 1994; 12(3)∶475.
  6. 6. Heiss MM, Allgayer H, Gruetzner KU, et al. Individual development and uPAreceptor expression of disseminated tumour cells in bone marrow: a reference to early systemic disease in solid cancer [Ｊ]. Nat Med, 1995; 1(10)∶1035.
  7. 7. Gala JL, Loric S. Sensitivity of specificity of reverse transcriptasepolymerase chain reaction assays: the real challenge for molecular staging of prostatic carcinomas [Ｊ]. Int J Cancer, 1998; 77(1)∶161.
  8. 8. Wolfgang H, Stefan A, Loening. Detection of illegitimate transcripts of prostatespecific antigen mRNA in blood by reverse transcriptionpolymerase chain reaction [Ｊ]. Int J cancer, 1998; 77(1)∶164.
  9. 9. 駱成玉，李世擁. 大腸癌患者外周血癌細(xì)胞檢測臨床意義 [Ｊ]. 中華實(shí)驗(yàn)外科雜志, 1999; 16（3）∶19.