目的 研究尿激酶型纖溶酶原激活劑(uPA) mRNA在胃癌組織中的表達(dá)及其意義。
方法 應(yīng)用熒光定量RTPCR方法,分別檢測48例胃癌及其癌旁組織中uPA mRNA的表達(dá),并結(jié)合其臨床病理特征進(jìn)行分析。
結(jié)果 48例胃癌組織及癌旁組織中,uPA mRNA表達(dá)陽性率分別為83.3%和25.0%。胃癌伴有淋巴結(jié)轉(zhuǎn)移和無淋巴結(jié)轉(zhuǎn)移者中,uPA mRNA表達(dá)陽性率分別為93.8%和62.5%,且uPA mRNA表達(dá)與胃癌浸潤程度呈正相關(guān)。
結(jié)論 uPA mRNA表達(dá)可作為判斷胃癌惡性程度及預(yù)后的指標(biāo)之一。
引用本文: 熊俊,鄭啟昌,宋自芳. 尿激酶型纖溶酶原激活劑mRNA在胃癌組織中的表達(dá)及其意義. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(2): 116-118. doi: 復(fù)制
版權(quán)信息: ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有,未經(jīng)授權(quán)不得轉(zhuǎn)載、改編
1. | Astedt B, Holmberg L. Immunological identity of urokinase and ovarian carcinoma plasminogen activator released in tissue culture [J]. Nature, 1976; 261(5561)∶595. |
2. | Chambers SK, Gertz RE Jr, Ivins CM, et al. The significance of urokinasetype plasminogen activator, its inhibitors, and its receptor in ascites of patients with epithelial ovarian cancer [J]. Cancer, 1995; 75(7)∶1627. |
3. | Konecny G, Untch M, Arboleda J, et al. Her2/neu and urokinasetype plasminogen activator and its inhibitor in breast cancer [J]. Clin Cancer Res, 2001; 7(8)∶2448. |
4. | Ito H, Yonemura Y, Fujita H, et al. Prognostic relevance of urokinasetype plasminogen activator (uPA) and plasminogen activator inhibitors PAI1 and PAI2 in gastric cancer [J]. Virchows Arch, 1996; 427(5)∶487. |
5. | Heiss MM, Babic R, Allgayer H, et al. Tumorassociated proteolysis and prognosis: new functional risk factors in gastric cancer defined by the urokinasetype plasminogen activator system [J]. J Clin Oncol, 1995; 13(8)∶2084. |
6. | Hsu DW, Efird JT, HedleyWhyte ET. Prognostic role of urokinasetype plasminogen activator in human gliomas [J]. Am J Pathol, 1995; 147(1)∶114. |
7. | Duggan C, Maguire T, McDermott E, et al. Urokinase plasminogen activator and urokinase plasminogen activator receptor in breast cancer [J]. Int J Cancer, 1995; 61(5)∶597. |
8. | Schneider J, Pollan M, Tejerina A, et al. Accumulation of uPAPAI1 complexes inside the tumour cells is associated with axillary nodal invasion in progesteronereceptorpositive early breast cancer [J]. Br J Cancer, 2003; 88(1)∶96. |
9. | Spyratos F, Bouchet C, Tozlu S, et al. Prognostic value of uPA, PAI1 and PAI2 mRNA expression in primary breast cancer [J]. Anticancer Res, 2002; 22(5)∶2997. |
10. | Casella R, Shariat SF, Monoski MA, et al. Urinary levels of urokinasetype plasminogen activator and its receptor in the detection of bladder carcinoma [J]. Cancer, 2002; 95(12)∶2494. |
11. | Tanaka N, Fukao H, Ueshima S, et al. Plasminogen activator inhibitor 1 in human carcinoma tissues [J]. Int J Cancer, 1991; 48(4)∶481. |
12. | Heid CA, Stevens J, Livak KJ, et al. Real time quantitative PCR [J]. Genome Res, 1996; 6(10)∶986. |
13. | Swan DC, Tucker RA, Holloway BP, et al. A sensitive, typespecific, fluorogenic probe assay for detection of human papillomavirus DNA [J]. J Clin Microbiol, 1997; 35(4)∶886. |
- 1. Astedt B, Holmberg L. Immunological identity of urokinase and ovarian carcinoma plasminogen activator released in tissue culture [J]. Nature, 1976; 261(5561)∶595.
- 2. Chambers SK, Gertz RE Jr, Ivins CM, et al. The significance of urokinasetype plasminogen activator, its inhibitors, and its receptor in ascites of patients with epithelial ovarian cancer [J]. Cancer, 1995; 75(7)∶1627.
- 3. Konecny G, Untch M, Arboleda J, et al. Her2/neu and urokinasetype plasminogen activator and its inhibitor in breast cancer [J]. Clin Cancer Res, 2001; 7(8)∶2448.
- 4. Ito H, Yonemura Y, Fujita H, et al. Prognostic relevance of urokinasetype plasminogen activator (uPA) and plasminogen activator inhibitors PAI1 and PAI2 in gastric cancer [J]. Virchows Arch, 1996; 427(5)∶487.
- 5. Heiss MM, Babic R, Allgayer H, et al. Tumorassociated proteolysis and prognosis: new functional risk factors in gastric cancer defined by the urokinasetype plasminogen activator system [J]. J Clin Oncol, 1995; 13(8)∶2084.
- 6. Hsu DW, Efird JT, HedleyWhyte ET. Prognostic role of urokinasetype plasminogen activator in human gliomas [J]. Am J Pathol, 1995; 147(1)∶114.
- 7. Duggan C, Maguire T, McDermott E, et al. Urokinase plasminogen activator and urokinase plasminogen activator receptor in breast cancer [J]. Int J Cancer, 1995; 61(5)∶597.
- 8. Schneider J, Pollan M, Tejerina A, et al. Accumulation of uPAPAI1 complexes inside the tumour cells is associated with axillary nodal invasion in progesteronereceptorpositive early breast cancer [J]. Br J Cancer, 2003; 88(1)∶96.
- 9. Spyratos F, Bouchet C, Tozlu S, et al. Prognostic value of uPA, PAI1 and PAI2 mRNA expression in primary breast cancer [J]. Anticancer Res, 2002; 22(5)∶2997.
- 10. Casella R, Shariat SF, Monoski MA, et al. Urinary levels of urokinasetype plasminogen activator and its receptor in the detection of bladder carcinoma [J]. Cancer, 2002; 95(12)∶2494.
- 11. Tanaka N, Fukao H, Ueshima S, et al. Plasminogen activator inhibitor 1 in human carcinoma tissues [J]. Int J Cancer, 1991; 48(4)∶481.
- 12. Heid CA, Stevens J, Livak KJ, et al. Real time quantitative PCR [J]. Genome Res, 1996; 6(10)∶986.
- 13. Swan DC, Tucker RA, Holloway BP, et al. A sensitive, typespecific, fluorogenic probe assay for detection of human papillomavirus DNA [J]. J Clin Microbiol, 1997; 35(4)∶886.