• 1.山東省立醫(yī)院普外科（濟(jì)南250021）;;
  • 2.山東省鄄城縣人民醫(yī)院外科（鄄城274600）;

【摘要】目的探討直腸癌中p27的表達(dá)和血清轉(zhuǎn)化生長(zhǎng)因子β1（TGF-β1）的水平，以及TGF-β1對(duì)p27的調(diào)控關(guān)系。
方法對(duì)37例直腸癌、22例直腸腺瘤和19例正常對(duì)照者用免疫組化二步法檢測(cè)其p27的表達(dá)情況，用酶聯(lián)免疫吸附法（ELISA）測(cè)定血清TGF-β1水平。
結(jié)果p27在正常直腸組織和直腸腺瘤組織中呈高表達(dá)，其表達(dá)陽(yáng)性率分別為89.47%和90.91%，陽(yáng)性表達(dá)定位于細(xì)胞核； 直腸癌組織中p27表達(dá)陽(yáng)性率降為64.87%，與前兩者比較，差異有統(tǒng)計(jì)學(xué)意義（P=0.025），且伴有p27細(xì)胞漿表達(dá)者占45.83%。血清TGF-β1在正常對(duì)照組的表達(dá)陽(yáng)性率為21.05%，直腸腺瘤組為27.27%，而直腸癌組則升至51.35%（P=0.045）。p27的表達(dá)與直腸癌的分化程度、淋巴結(jié)轉(zhuǎn)移和浸潤(rùn)深度有關(guān)； 血清TGF-β1表達(dá)水平與直腸癌的淋巴結(jié)轉(zhuǎn)移、浸潤(rùn)深度和CEA水平有關(guān)。血清TGF-β1陰性組中p27表達(dá)陽(yáng)性率為88.89%，血清TGF-β1陽(yáng)性組中p27表達(dá)陽(yáng)性率降為42.11%，差異有顯著性意義（MantelHaenszel χ2=6.755，P=0.009）。
結(jié)論p27對(duì)于判斷直腸癌的惡性程度和預(yù)后有指導(dǎo)作用，血清TGF-β1可作為直腸癌的輔助診斷指標(biāo)，TGF-β1可下調(diào)直腸癌中p27的蛋白表達(dá)。

引用本文： 靖昌慶,李樂(lè)平,李昭,吳泰璜,穆慶嶺. 直腸癌p27基因表達(dá)及血清轉(zhuǎn)化生長(zhǎng)因子β1水平的變化. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(1): 33-36. doi: 復(fù)制

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  3. 3. Singh SP, Lipman J, Goldman H, et al. Loss or altered subcellular localization of p27 in Barrett’s associated adenocarcinoma [Ｊ]. Cancer Res， 1998; 58(8)∶1730.
  4. 4. Palmqvist R, Stenling R, Oberg A, et al. Prognostic significance of p27(Kip1) expression in colorectal cancer: a clinicopathological characterization [Ｊ]. J Pathol， 1999; 188(1)∶18.
  5. 5. Pasche B. Role of transforming growth factor beta in cancer [Ｊ]. J Cell Physiol， 2001; 186(2)∶153.
  6. 6. Fan G, Ma X, Wong PY, et al. p53 dephosphorylation and p21(Cip1/Waf1) translocation correlate with caspase3 activation in TGFbeta1induced apoptosis of HuH7 cells [Ｊ]. Apoptosis， 2004; 9(2)∶211.
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