• 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院甲狀腺、乳腺外科中心(武漢 430022);

【摘要】目的 探討雌激素水平對(duì)三苯氧胺(tamoxifen, TAM)抑制乳腺癌細(xì)胞MCF7(ER陽(yáng)性)生長(zhǎng)的影響。 方法 應(yīng)用不同濃度的雌二醇(estradiol, E2)和10 μmol/L濃度的TAM作用于MCF7細(xì)胞,應(yīng)用四甲基偶氮唑藍(lán)(MTT)比色法分析其對(duì)細(xì)胞生長(zhǎng)的抑制作用。 結(jié)果 用1×10-12 mol/L的 E2開(kāi)始刺激MCF7細(xì)胞生長(zhǎng),到1×10-9 mol/L時(shí)細(xì)胞生長(zhǎng)達(dá)到平臺(tái)期。10 μmol/L的TAM可以明顯抑制細(xì)胞生長(zhǎng),不同濃度的E2可以影響TAM對(duì)MCF7細(xì)胞生長(zhǎng)的抑制作用。當(dāng)E2濃度達(dá)到1×10-8 mol/L 時(shí),TAM對(duì)細(xì)胞生長(zhǎng)的抑制作用最弱。 結(jié)論 E2是乳腺癌的危險(xiǎn)因素,腫瘤細(xì)胞周圍E2的濃度可以影響TAM的療效。

引用本文: 韓明強(qiáng),黃韜,田元. 雌激素水平對(duì)三苯氧胺抑制乳腺癌細(xì)胞的體外研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(6): 589-590下轉(zhuǎn)594. doi: 復(fù)制

版權(quán)信息: ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有,未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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