• 1.華中科技大學同濟醫(yī)學院附屬協(xié)和醫(yī)院普外科 (武漢430022);;
  • 2.華中科技大學同濟醫(yī)學院附屬協(xié)和醫(yī)院急診外科;;
  • 3.華中科技大學同濟醫(yī)學院附屬協(xié)和醫(yī)院兒科;

目的　觀察氯化釓(GdCl3)對內(nèi)毒素刺激后的小鼠巨噬細胞來源的細胞系RAW264.7細胞Toll樣受體(TLRs)表達的影響。方法　 RAW264.7細胞分為空白組、內(nèi)毒素處理組（LPS組）及GdCl3處理組（GdCl3組），采用流式細胞儀檢測TLR2/4蛋白表達情況； 用逆轉錄PCR（RT-PCR）法分析細胞中TLR2/4 mRNA表達的變化； 用ELISA檢測細胞培養(yǎng)上清液中TNF-α的水平。結果　與LPS組相比，不同濃度的GdCl3作用于RAW264.7細胞后，其TLR2/4蛋白和基因的表達以及TNF-α的表達水平均明顯下降，在觀察濃度范圍內(nèi)以2 000 μmol/L時最明顯，TLR2/4蛋白： 200 μmol/L時為（70.2±1.28）％/（66.7±2.59）％，400 μmol/L時為（64.9±1.43）％/（60.4±1.25）％，2 000 μmol/L時為（47.4±0.98）％/（32.1±0.74）％，其與 LPS組的（94.4±1.76）％/（95.7±0.87）％比較，P＜0.01； TLR2/4 mRNA （A值）： 200 μmol/L時為（76.42±2.76）/（101.72±3.14）， 400 μmol/L時為（75.60±3.76）/（89.65±5.17），2 000 μmol/L時為（64.22±4.67）/（78.44±4.88），其與 LPS組的（127.64±3.25）/（119.82±5.59）比較，P＜0.05, P＜0.01； TNF-α： 200 μmol/L時為 （2 540±77） pg/ml， 400 μmol/L時為 （2 041±106） pg/ml，2 000 μmol/L時為（1 020±220） pg/ml ，其與 LPS組的（4 688±127） pg/ml比較，P＜0.01。結論　GdCl3能明顯抑制內(nèi)毒素引起的RAW264.7細胞Toll樣受體的表達及相應炎癥因子的生成。

引用本文： 徐建波,吳河水,張進祥,王琳,金欽文,田元,張景輝,張磊. 氯化釓對內(nèi)毒素刺激后小鼠巨噬細胞Toll樣受體表達的影響. 中國普外基礎與臨床雜志, 2006, 13(6): 681-684. doi: 復制

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  2. 2. 　Wu HS, Zhang JX, Wang L, et al. Toll-like receptor 4 inv-olvement in hepatic ischemia/reperfusion injury in mice [Ｊ]. Hepatobiliary Pancreat Dis Int， 2004; 3(2)∶250.
  3. 3. 　Medzhitov R. Toll-like receptors and innate immunity [Ｊ]. Nat Rev Immunol， 2001; 1(2)∶135.
  4. 4. 　Schauer RJ, Bilzer M, Kalmuk S, et al. Microcirculatory failure after rat liver transplantation is related to Kupffer cell-derived oxidant stress but not involved in early graft dysfunction [Ｊ]. Transplantation， 2001; 72(10)∶1692.
  5. 5. 　Shibuya H, Ohkohchi N, Seya K, et al. Kupffer cells generate superoxide anions and modulate reperfusion injury in rat livers after cold preservation [Ｊ]. Hepatology， 1997； 25(2)∶35.
  6. 6. 　Dekai Zhang, Guolong Zhang, Matthew S, et al. A toll-like receptor that prevents infection by uropathogenic bacteria[Ｊ]. Science， 2004; 303（5633）∶1522.
  7. 7. 　Aliprantis AO, Yang RB, Mark MR, et al. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2 [Ｊ]. Science， 1999； 285 (5428)∶736.
  8. 8. 　吳河水， 王　峰， 王　琳， 等. 小鼠肝缺血/再灌注損傷時肝臟Kupffer細胞中TLR2的表達 [Ｊ]. 中國普通外科雜志, 2004； 13(8)∶591.
  9. 9. 　Musikacharoen T, Matsuguchi T, Kikuchi T, et al. NF-kappa B and STAT5 play important roles in the regulation of mouse Toll-like receptor 2 gene expression [Ｊ]. J Immunol, 2001； 166（7）∶4516.
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