• 1.成都軍區(qū)總醫(yī)院全軍普外中心(成都610083);;
  • 2.廣州珠江醫(yī)院肝膽外科(廣州510282);

目的  研究原發(fā)性肝細(xì)胞癌發(fā)生、發(fā)展過(guò)程中肝組織增殖細(xì)胞核抗原(proliferating cell nuclear antigen, PCNA)的動(dòng)態(tài)演變規(guī)律。
方法  將60只SD大鼠按隨機(jī)數(shù)字表法隨機(jī)分為對(duì)照組(20只)和實(shí)驗(yàn)組(40只)。用化學(xué)致癌劑3,3′-二氨基聯(lián)苯胺(3,3′-diaminobenzidine,DAB)誘發(fā)SD大鼠肝癌,采用免疫組織化學(xué)方法連續(xù)監(jiān)測(cè)各時(shí)相肝組織中PCNA的表達(dá),光鏡下觀察肝組織的病理變化。
結(jié)果  本實(shí)驗(yàn)的誘癌過(guò)程可分為炎性變期、增生纖維化期和癌變期3個(gè)階段; PCNA的表達(dá)最早出現(xiàn)于炎性變?cè)缙趨R管區(qū)的卵圓細(xì)胞中,并在匯管區(qū)呈區(qū)域性過(guò)表達(dá)分布; 炎性變中期,PCNA表達(dá)陽(yáng)性率達(dá)高峰,隨后下降,在癌變期又升高,在整個(gè)病理演變過(guò)程中其表達(dá)具波動(dòng)性。
結(jié)論  在致癌因素的存在下,PCNA首先表達(dá)于卵圓細(xì)胞; PCNA表達(dá)的動(dòng)態(tài)演變對(duì)肝癌的發(fā)生有一定的預(yù)警作用。

引用本文: 龔加慶,方馳華. 大鼠肝癌發(fā)生發(fā)展過(guò)程中肝組織增殖細(xì)胞核抗原動(dòng)態(tài)演變規(guī)律. 中國(guó)普外基礎(chǔ)與臨床雜志, 2007, 14(1): 11-14. doi: 復(fù)制

版權(quán)信息: ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有,未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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  2. 2.  Chung JK, Lee T, Jung IM, et al. Expression of cell cycle regulators during smooth muscle cell proliferation after balloon catheter injury of rat artery [J]. J Korean Med Sci, 2004; 19(3)∶327.
  3. 3.  Guettier C. Which stem cells for adult liver? [J].Ann Pathol, 2005; 25(1)∶33.
  4. 4.  Oh BK, Lee CH, Park C, et al. Telomerase regulation and progressive telomere shortening of rat hepatic stemlike epithelial cells during in vitro aging [J]. Exp Cell Res, 2004; 298(2)∶445.
  5. 5.  Hatch HM, Zheng D, Jorgensen ML, et al. SDF1alpha/CXCR4: a mechanism for hepatic oval cell activation and bone marrow stem cell recruitment to the injured liver of rats [J]. Cloning Stem Cells, 2002; 4(4)∶339.
  6. 6.  龔加慶, 方馳華, 李雅. 肝卵圓細(xì)胞在進(jìn)行性肝損傷過(guò)程中分布及遷移的實(shí)驗(yàn)研究 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2004; 11(5)∶389.
  7. 7.  韋偉, 龔建平, 裘法祖. 正常和異常肝組織增生中細(xì)胞增殖與凋亡的關(guān)系 [J]. 第三軍醫(yī)大學(xué)學(xué)報(bào), 2001; 18(2)∶156.
  8. 8.  Fiegel HC, Gluer S, Roth B, et al. Stemlike cells in human hepatoblastoma [J]. J Histochem Cytochem, 2004; 52(11)∶1495.