• 第三軍醫(yī)大學(xué)野戰(zhàn)外科研究所肝膽外科( 重慶 400042);

【摘要】　目的　研究反義核酸技術(shù)封閉谷氨酰胺酶( GA) 基因?qū)β闶篌w內(nèi)胃癌細胞凋亡作用的影響。方法 將攜帶GA 反義基因的質(zhì)粒轉(zhuǎn)染胃癌細胞SGC27901 ,并將成功轉(zhuǎn)染的胃癌細胞接種于裸鼠皮下,獲得裸鼠胃癌移植瘤模型,通過TUNEL 技術(shù)檢測胃癌細胞凋亡的凋亡指數(shù); RT2PCR 技術(shù)檢測移植瘤細胞內(nèi)GA mRNA 的含量。結(jié)果　GA 反義基因質(zhì)粒載體成功轉(zhuǎn)染胃癌細胞后,腫瘤生長速度減慢,癌細胞凋亡增加,腫瘤細胞內(nèi)GA mRNA的含量減少。結(jié)論　GA 反義基因可抑制GA 基因的表達,明顯促進胃癌細胞凋亡。

引用本文： 劉孟剛,楊俊濤,劉宏鳴,袁濤,顧紅光. GA 反義基因?qū)β闶笪赴┮浦擦黾毎蛲龅挠绊? 中國普外基礎(chǔ)與臨床雜志, 2008, 15(1): 4-7. doi: 復(fù)制

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1. Medina MA,Sanchez-Jiménez F,Mrquez J,et al.Relevance of glutamine metabolism to tumor cell growth[J].Mol Cell Biochem,1992; 113(1)∶1.
2. Medina MA.Glutamine and cancer[J].J Nutr,2001; 131(9 Suppl)∶2539S.
3. 劉宏鳴,楊俊濤,顧紅光.大鼠谷氨酰胺酶反義真核表達載體的構(gòu)建及鑒定[J].醫(yī)學(xué)研究生學(xué)報,2003; 16(7)∶487.
4. Simpson DA,Feeney S,Boyle C,et al.Retinal VEGF mRNA measured by SYBR green Ⅰ fluorescence:A versatile approach to quantitative PCR[J].Mol Vis,2000; 6∶178.
5. Zhang WW,Fang X,Branch CD,et al.Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis[J].Biotechniques,1993; 15(5)∶868.
6. Medina MA,Sánchez-Jiménez F,Quesada AR,et al.Effect of palmitate,acetate and glucose on glutamine metabolism in Ehrlich ascites tumor cells[J].Biochimie,1988; 70(6)∶833.
7. Pérez-Gómez C,Campos-Sandoval JA,Alonso FJ,et al.Co-expression of glutaminase K and L isoenzymes in human tumour cells[J].Biochem J,2005; 386(Pt 3)∶535.
8. Zacharias DP,Lima MM,Souza AL Jr,et al.Human cutaneous melanoma expresses a significant phosphate-dependent glutaminase activity:a comparison with the surrounding skin of the same patient[J].Cell Biochem Funct,2003; 21(1)∶81.
9. Turner A,McGivan JD.Glutaminase isoform expression in cell lines derived from human colorectal adenomas and carcinomas[J].Biochem J,2003; 370(Pt 2)∶403.
10. Aledo JC,Segura JA,Medina MA,et al.Phosphate-activated glutaminase expression during tumor development[J].FEBS Lett,1994; 341(1)∶39.
11. Aledo JC,de Pedro E,Gómez-Fabre PM,et al.Changes in mRNAs for enzymes of glutamine metabolism in the tumor-bearing mouse[J].Anticancer Res,2000; 20(3A)∶1463.
12. 陳道達,鄭海.胃癌的基因治療現(xiàn)狀、問題和展望[J].中國普外基礎(chǔ)與臨床雜志,2002; 9(3)∶141.
13. 朱金明,余佩武.胃癌生物治療現(xiàn)狀與展望[J].中國普外基礎(chǔ)與臨床雜志,2004; 11(2)∶127.
14. 陸芬英,張尤歷.胃癌基因治療研究進展[J].醫(yī)學(xué)綜述,2007; 13(3)∶191.
15. 劉孟剛,楊俊濤,劉宏鳴.GA反義基因?qū)β闶笪赴┮浦擦龅囊种谱饔肹J].消化外科,2005; 11(4)∶430.
16. Fulda S,Debatin KM.Death receptor signaling in cancer therapy[J].Curr Med Chem Anticancer Agents,2003; 3(4)∶253.
17. Oka T,Yoshikawa M,Kobayashi T,et al.Effects of antisense oligodeoxynucleotides against opioid receptors on the receptor mRNA contents[J].Life Sci,2001; 68(19-20)∶2221.
18. Franek F,Fismolova I,Eckschlager T.Antiapoptotic and proapoptotic action of various amino acids and analogs in starving MOLT-4 cells[J].Arch Biochem Biophys,2002; 398(1)∶141.
19. Ghosh S,Roy S,Banerjee M,et al.Modulation of tumor induced angiogenesis in Ehrlich ascites tumor[J].J Exp Clin Cancer Res,2004; 23(4)∶681.
20. Lora J,Alonso FJ,Segura JA,et al.Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells[J].Eur J Biochem,2004; 271(21)∶4298.
  1. 1. Medina MA,Sanchez-Jiménez F,Mrquez J,et al.Relevance of glutamine metabolism to tumor cell growth[J].Mol Cell Biochem,1992; 113(1)∶1.
  2. 2. Medina MA.Glutamine and cancer[J].J Nutr,2001; 131(9 Suppl)∶2539S.
  3. 3. 劉宏鳴,楊俊濤,顧紅光.大鼠谷氨酰胺酶反義真核表達載體的構(gòu)建及鑒定[J].醫(yī)學(xué)研究生學(xué)報,2003; 16(7)∶487.
  4. 4. Simpson DA,Feeney S,Boyle C,et al.Retinal VEGF mRNA measured by SYBR green Ⅰ fluorescence:A versatile approach to quantitative PCR[J].Mol Vis,2000; 6∶178.
  5. 5. Zhang WW,Fang X,Branch CD,et al.Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis[J].Biotechniques,1993; 15(5)∶868.
  6. 6. Medina MA,Sánchez-Jiménez F,Quesada AR,et al.Effect of palmitate,acetate and glucose on glutamine metabolism in Ehrlich ascites tumor cells[J].Biochimie,1988; 70(6)∶833.
  7. 7. Pérez-Gómez C,Campos-Sandoval JA,Alonso FJ,et al.Co-expression of glutaminase K and L isoenzymes in human tumour cells[J].Biochem J,2005; 386(Pt 3)∶535.
  8. 8. Zacharias DP,Lima MM,Souza AL Jr,et al.Human cutaneous melanoma expresses a significant phosphate-dependent glutaminase activity:a comparison with the surrounding skin of the same patient[J].Cell Biochem Funct,2003; 21(1)∶81.
  9. 9. Turner A,McGivan JD.Glutaminase isoform expression in cell lines derived from human colorectal adenomas and carcinomas[J].Biochem J,2003; 370(Pt 2)∶403.
  10. 10. Aledo JC,Segura JA,Medina MA,et al.Phosphate-activated glutaminase expression during tumor development[J].FEBS Lett,1994; 341(1)∶39.
  11. 11. Aledo JC,de Pedro E,Gómez-Fabre PM,et al.Changes in mRNAs for enzymes of glutamine metabolism in the tumor-bearing mouse[J].Anticancer Res,2000; 20(3A)∶1463.
  12. 12. 陳道達,鄭海.胃癌的基因治療現(xiàn)狀、問題和展望[J].中國普外基礎(chǔ)與臨床雜志,2002; 9(3)∶141.
  13. 13. 朱金明,余佩武.胃癌生物治療現(xiàn)狀與展望[J].中國普外基礎(chǔ)與臨床雜志,2004; 11(2)∶127.
  14. 14. 陸芬英,張尤歷.胃癌基因治療研究進展[J].醫(yī)學(xué)綜述,2007; 13(3)∶191.
  15. 15. 劉孟剛,楊俊濤,劉宏鳴.GA反義基因?qū)β闶笪赴┮浦擦龅囊种谱饔肹J].消化外科,2005; 11(4)∶430.
  16. 16. Fulda S,Debatin KM.Death receptor signaling in cancer therapy[J].Curr Med Chem Anticancer Agents,2003; 3(4)∶253.
  17. 17. Oka T,Yoshikawa M,Kobayashi T,et al.Effects of antisense oligodeoxynucleotides against opioid receptors on the receptor mRNA contents[J].Life Sci,2001; 68(19-20)∶2221.
  18. 18. Franek F,Fismolova I,Eckschlager T.Antiapoptotic and proapoptotic action of various amino acids and analogs in starving MOLT-4 cells[J].Arch Biochem Biophys,2002; 398(1)∶141.
  19. 19. Ghosh S,Roy S,Banerjee M,et al.Modulation of tumor induced angiogenesis in Ehrlich ascites tumor[J].J Exp Clin Cancer Res,2004; 23(4)∶681.
  20. 20. Lora J,Alonso FJ,Segura JA,et al.Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells[J].Eur J Biochem,2004; 271(21)∶4298.