• 華中科技大學(xué)同濟醫(yī)學(xué)院附屬同濟醫(yī)院膽胰外科（武漢430030）;

目的 在膽管結(jié)扎（BDL）模型中觀察神經(jīng)生長因子（NGF）及其高親和力受體酪氨酸激酶A（TrkA）在增殖的膽管周圍結(jié)締組織中的表達(dá)以及梗阻緩解后其表達(dá)的變化。
方法 46只SD雌性大鼠，隨機分為對照組（ n =6）和BDL組（ n =40），建立大鼠阻塞性黃疸模型，取肝十二指腸韌帶，采用免疫組化方法檢測NGF和TrkA在膽管結(jié)締組織中的表達(dá)和分布。同時左心室采血檢測血清總膽紅素和谷丙轉(zhuǎn)氨酶水平。
結(jié)果 BDL組膽總管結(jié)扎后，大鼠膽紅素水平于早期升高： 3 d時有明顯升高，持續(xù)到第14 d； 后期下降： 第21 d和28 d時膽紅素水平已基本降至正常。BDL組膽管壁直徑明顯增加，與結(jié)締組織細(xì)胞增殖分化相平行。NGF及TrkA受體在膽管結(jié)締組織細(xì)胞和炎性浸潤細(xì)胞胞膜和胞漿中表達(dá)，其表達(dá)的變化與膽紅素水平的變化趨勢基本一致。
結(jié)論 NGF和 TrkA受 體對膽管周圍結(jié)締組織的增殖、分化有調(diào)節(jié)作用，可能參與了膽管瘢痕的形成過程，淤膽狀態(tài)的緩解在短期內(nèi)可能對膽管瘢痕形成無逆轉(zhuǎn)作用。

引用本文： 汪雷,鄧小強,舒藝,鄭順貞,鄒聲泉. 神經(jīng)生長因子及其受體在膽管結(jié)締組織中的表達(dá)及意義. 中國普外基礎(chǔ)與臨床雜志, 2009, 16(1): 39-43. doi: 復(fù)制

1. Guyot C, Combe C, Desmoulière A. The common bile duct ligation in rat: A relevant in vivo model to study the role of mechanical stress on cell and matrix behaviour [Ｊ] . Histochem Cell Biol, 2006; 126(4)：517.
2. Gigliozzi A, Alpini G, Baroni GS, et al . Nerve growth factor modulates the proliferative capacity of the intrahepatic biliary epithelium in experimental cholestasis [Ｊ] . Gastroenterology, 2004; 127(4)：1198.
3. Aloe L, Bracci-Laudiero L, Bonini S, et al . The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases [Ｊ] . Allergy, 1997; 52(9)：883.
4. Freund-Michel V, Frossard N. The nerve growth factor and its receptors in airway inflammatory diseases [Ｊ] . Pharmacol Ther, 2008; 117(1)：52.
5. Alvaro D, Mancino MG, Onori P, et al . Estrogens and the pathophysiology of the biliary tree [Ｊ] . World J Gastroenterol, 2006; 12(22)：3537.
6. Takahashi H, Uno S, Watanabe Y, et al . Expression of nerve growth factor-induced type 1 plasminogen activator inhibitor (PAI-1) mRNA is inhibited by genistein and wortmannin [Ｊ] . Neuroreport, 2000; 11(5)：1111.
7. Olgart Hglund C, de Blay F, Oster JP, et al . Nerve growth factor levels and localisation in human asthmatic bronchi [Ｊ] . Eur Respir J, 2002; 20(5)：1110.
8. Freund-Michel V, Bertrand C, Frossard N. TrkA signalling pathways in human airway smooth muscle cell proliferation [Ｊ] . Cell Signal, 2006; 18(5)：621.
9. Levi-Montalcini R. The nerve growth factor 35 years later [Ｊ] . Science, 1987; 237(4819)：1154.
10. 尹蘭寧， 侯治軍， 宋愛琳， 等. 良性梗阻性黃疸患者血清中IGF-1變化的意義 [Ｊ] . 中國普外基礎(chǔ)與臨床雜志， 2005; 12(4)：349.
11. 劉雙海， 蔡景修， 顧紅光， 等. 炎性狹窄膽管壁中成纖維細(xì)胞表型改變及增殖的意義 [Ｊ] . 中國普外基礎(chǔ)與臨床雜志， 2000; 7(2)：69.
12. Kountouras J, Scheuer PJ, Billing BH. Effect of prolonged bile duct obstruction in the rat on hepatic transport of bilirubin [Ｊ] . Clin Sci (Lond), 1985; 68(3)：341.
13. 尹珊珊， 王寶恩， 賈繼東， 等. 膽管阻塞性肝纖維化模型的建立和改進 [Ｊ] . 中華肝臟病雜志， 2002； 10(5)：385.
14. Nithya M, Suguna L, Rose C. The effect of nerve growth factor on the early responses during the process of wound healing [Ｊ] . Biochim Biophys Acta, 2003; 1620(1-3)：25.
15. Micera A, Lambiase A, Stampachiacchiere B, et al . Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate [Ｊ] . Cytokine Growth Factor Rev, 2007; 18(3-4)：245.
  1. 1. Guyot C, Combe C, Desmoulière A. The common bile duct ligation in rat: A relevant in vivo model to study the role of mechanical stress on cell and matrix behaviour [Ｊ] . Histochem Cell Biol, 2006; 126(4)：517.
  2. 2. Gigliozzi A, Alpini G, Baroni GS, et al . Nerve growth factor modulates the proliferative capacity of the intrahepatic biliary epithelium in experimental cholestasis [Ｊ] . Gastroenterology, 2004; 127(4)：1198.
  3. 3. Aloe L, Bracci-Laudiero L, Bonini S, et al . The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases [Ｊ] . Allergy, 1997; 52(9)：883.
  4. 4. Freund-Michel V, Frossard N. The nerve growth factor and its receptors in airway inflammatory diseases [Ｊ] . Pharmacol Ther, 2008; 117(1)：52.
  5. 5. Alvaro D, Mancino MG, Onori P, et al . Estrogens and the pathophysiology of the biliary tree [Ｊ] . World J Gastroenterol, 2006; 12(22)：3537.
  6. 6. Takahashi H, Uno S, Watanabe Y, et al . Expression of nerve growth factor-induced type 1 plasminogen activator inhibitor (PAI-1) mRNA is inhibited by genistein and wortmannin [Ｊ] . Neuroreport, 2000; 11(5)：1111.
  7. 7. Olgart Hglund C, de Blay F, Oster JP, et al . Nerve growth factor levels and localisation in human asthmatic bronchi [Ｊ] . Eur Respir J, 2002; 20(5)：1110.
  8. 8. Freund-Michel V, Bertrand C, Frossard N. TrkA signalling pathways in human airway smooth muscle cell proliferation [Ｊ] . Cell Signal, 2006; 18(5)：621.
  9. 9. Levi-Montalcini R. The nerve growth factor 35 years later [Ｊ] . Science, 1987; 237(4819)：1154.
  10. 10. 尹蘭寧， 侯治軍， 宋愛琳， 等. 良性梗阻性黃疸患者血清中IGF-1變化的意義 [Ｊ] . 中國普外基礎(chǔ)與臨床雜志， 2005; 12(4)：349.
  11. 11. 劉雙海， 蔡景修， 顧紅光， 等. 炎性狹窄膽管壁中成纖維細(xì)胞表型改變及增殖的意義 [Ｊ] . 中國普外基礎(chǔ)與臨床雜志， 2000; 7(2)：69.
  12. 12. Kountouras J, Scheuer PJ, Billing BH. Effect of prolonged bile duct obstruction in the rat on hepatic transport of bilirubin [Ｊ] . Clin Sci (Lond), 1985; 68(3)：341.
  13. 13. 尹珊珊， 王寶恩， 賈繼東， 等. 膽管阻塞性肝纖維化模型的建立和改進 [Ｊ] . 中華肝臟病雜志， 2002； 10(5)：385.
  14. 14. Nithya M, Suguna L, Rose C. The effect of nerve growth factor on the early responses during the process of wound healing [Ｊ] . Biochim Biophys Acta, 2003; 1620(1-3)：25.
  15. 15. Micera A, Lambiase A, Stampachiacchiere B, et al . Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate [Ｊ] . Cytokine Growth Factor Rev, 2007; 18(3-4)：245.