• 1.中國醫(yī)科大學附屬第四醫(yī)院肝膽胰外科(遼寧沈陽 110032);;
  • 2.遼寧省腫瘤醫(yī)院肝膽胰外科(遼寧沈陽 110043);

目的研究胰頭癌患者外周血中CD4+CD25+調(diào)節(jié)性T細胞(Treg)比例及其在手術(shù)前、后的變化趨勢。方法采用流式細胞儀檢測胰頭癌患者及正常對照組外周血中CD4+CD25+調(diào)節(jié)性T細胞的數(shù)量,同時監(jiān)測CD4+/CD8+比值,并進行手術(shù)前、后的比較。結(jié)果胰頭癌患者術(shù)前外周血中CD4+CD25+和CD4+CD25high Treg所占比例較正常對照組高(P lt;0.05),術(shù)后則出現(xiàn)不同程度下降,以術(shù)后第3天下降最明顯(P lt;0.01,P lt;0.05); 胰頭癌患者術(shù)后CA199水平低于術(shù)前,以術(shù)后第14天下降明顯(P lt;0.05)。 CD4+CD25highTreg與CA199的變化趨勢大致相同。 胰頭癌患者術(shù)前CD4+/CD8+比值比正常對照組低(P lt;0.05),手術(shù)后進一步降低,于手術(shù)后第7天達最低(P lt;0.05)。結(jié)論胰十二指腸切除術(shù)可能有助于機體抗腫瘤免疫的恢復,胰頭癌患者圍手術(shù)期可作為免疫干預的重要窗口期,CD4+CD25+調(diào)節(jié)性T細胞可作為免疫干預的靶點。

引用本文: 林琳,梁健,馬作紅,郭大偉,王學范,付慶才,孫文郁,姜洪磊,金俊哲,姜曉峰. 胰十二指腸切除術(shù)對胰頭癌患者CD4+CD25+調(diào)節(jié)性T細胞的影響及臨床意義△. 中國普外基礎(chǔ)與臨床雜志, 2011, 18(1): 51-54. doi: 復制

版權(quán)信息: ?四川大學華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有,未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Michalski CW, Weitz J, Büchler MW. Surgery insight: surgical management of pancreatic cancer [J]. Nat Clin Pract Oncol, 2007, 4(9): 526535.
2. Sakaguchi S, Sakaguchi N, Asano M, et al. Immunologic selftolenrance maintained by actived T cells expressing Il2 receptor αchain (CD25). Breakdown of a single mechanism of selftolerance causes various autoimmune disease [J]. J Immunol, 1995, 155(3): 11511164.
3. Abdulamir AS, Kadhim HS, Hafidh RR, et al. Severity of asthma: the role of CD25+, CD30+, NFκB, and apoptotic markers [J]. J Investig Allergol Clin Immunol, 2009, 19(3): 218224.
4. Stephens LA, Malpass KH, Anderton SM, et al. Curing CNS autoimmune disease with myelinreactive Foxp3+ Treg [J]. Eur J Immunol, 2009, 39(4): 11081117.
5. Woo EY, Chu CS, Goletz TJ, et al. Regulatory CD4+CD25+ T cells in tumors from patients with earlystage nonsmall cell lung cancer and latestage ovarian cancer [J]. Cancer Res, 2001, 61(12): 47664772.
6. Sasada T, Kimura M, Yoshida Y, et al. CD4+CD25+ regulatory T cells in patients with gastrointestinal malignancies:possible involvement of regulatory T cells in dieases progression [J]. Cancer, 2003, 98(5): 10891099.
7. Omandy LA, Hillemann T, Wedemeyer H, et al. Increased populations of regulatory T cells in peripheral blood of patients with hepatocelluar carcinoma [J]. Cancer Res, 2005, 65(6): 24572464.
8. 劉俊田, 岳杰, 任秀寶, 等. 乳腺癌患者外周血CD4+CD25+調(diào)節(jié)性T細胞的檢測及意義 [J]. 中華腫瘤雜志, 2005, 27(7): 423425.
9. Liyanage UK, Moore TT, Joo HG, et al. Prevalence of regulatory T cells is increased in peripheral blood and tumor microenvironment of patients with pancreas or breast adenocarcinoma [J]. J Immunol, 2002, 169(5): 27562761.
10. Hiraoka N, Onozato K, Kosuge T, et al. Prevalence of FOXP3+ regulatory T cells increases during the progression of pancreatic ductal adenocarcinoma and its premalignant lesions [J]. Clin Cancer Res, 2006, 12(18): 54235434.
11. Kono K, Kawaida H, Takahashi A, et al. CD4+CD25high regulatory T cells increase with tumor stage in patient with gastric and esophageal cancers [J]. Cancer Immunol Immunother, 2006, 55(9): 10641071.
12. Peng L, Kiaergard J, Plautz GE, et al. Tumorinduced Lselectin high suppressor T cells mediate potent effector T cell blockade and cause failure of otherwise curative adoptive immunotherapy [J]. J Immunol, 2002, 169(9): 48114821.
13. 鄔玉輝, 申正堂, 海健, 等. 乳腺癌患者細胞免疫功能的檢測 [J]. 中國醫(yī)師雜志, 2001, 3(8): 627628.
14. Sharma S, Yang SC, Zhu L, et al. Tumor cyclooxygenase2/prostaglandin E2dependent promotion of FOXP3 expression and CD4+CD25+ T regulatory cell activities in lung cancer [J]. Cancer Res, 2005, 65(12): 52115220.
15. Valzasina B, Piconese S, Guiducci C, et al. Tumorinduced expansion of regulatory T cells by conversion of CD4+CD25- lymphocytes is thymus and proliferation independent [J]. Cancer Res, 2006, 66(8): 44884495.
16. Ghiringhelli F, Puig PE, Roux S, et al. Tumor cells conver immature myeloid dendritic cells into TGFβsecreting cells inducing CD4+CD25+ regulatory T cell proliferation [J]. J Exp Med, 2005, 202(7): 919929.
17. MooYoung TA, Larson JW, Belt BA, et al. Tumorderived TGFβ mediates conversion of CD4+Foxp3+ regulatory T Cells in a murine model of pancreas cancer [J]. J Immunother, 2009, 32(1): 1221.
18. Curiel J, Conkos G, Zou L, et al. Specifc recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival [J]. Nat Med, 2004,10(9): 942949.
19. Dannull J, Su Z, Rizzieri D, et al. Enhancement of vaccinemediated antitumor immunity in cancer patients after depletion of regulatory T cells [J]. J Clin Invest, 2005, 115(12): 36233633.
20. Nummer D, SuriPayer E, SchmitzWinnenthal H, et al. Role of tumor endothelium in CD4+CD25+ regulatory T cell infiltration of human pancreatic carcinoma [J]. J Natl Cancer Inst, 2007, 99(15): 11881199.
  1. 1. Michalski CW, Weitz J, Büchler MW. Surgery insight: surgical management of pancreatic cancer [J]. Nat Clin Pract Oncol, 2007, 4(9): 526535.
  2. 2. Sakaguchi S, Sakaguchi N, Asano M, et al. Immunologic selftolenrance maintained by actived T cells expressing Il2 receptor αchain (CD25). Breakdown of a single mechanism of selftolerance causes various autoimmune disease [J]. J Immunol, 1995, 155(3): 11511164.
  3. 3. Abdulamir AS, Kadhim HS, Hafidh RR, et al. Severity of asthma: the role of CD25+, CD30+, NFκB, and apoptotic markers [J]. J Investig Allergol Clin Immunol, 2009, 19(3): 218224.
  4. 4. Stephens LA, Malpass KH, Anderton SM, et al. Curing CNS autoimmune disease with myelinreactive Foxp3+ Treg [J]. Eur J Immunol, 2009, 39(4): 11081117.
  5. 5. Woo EY, Chu CS, Goletz TJ, et al. Regulatory CD4+CD25+ T cells in tumors from patients with earlystage nonsmall cell lung cancer and latestage ovarian cancer [J]. Cancer Res, 2001, 61(12): 47664772.
  6. 6. Sasada T, Kimura M, Yoshida Y, et al. CD4+CD25+ regulatory T cells in patients with gastrointestinal malignancies:possible involvement of regulatory T cells in dieases progression [J]. Cancer, 2003, 98(5): 10891099.
  7. 7. Omandy LA, Hillemann T, Wedemeyer H, et al. Increased populations of regulatory T cells in peripheral blood of patients with hepatocelluar carcinoma [J]. Cancer Res, 2005, 65(6): 24572464.
  8. 8. 劉俊田, 岳杰, 任秀寶, 等. 乳腺癌患者外周血CD4+CD25+調(diào)節(jié)性T細胞的檢測及意義 [J]. 中華腫瘤雜志, 2005, 27(7): 423425.
  9. 9. Liyanage UK, Moore TT, Joo HG, et al. Prevalence of regulatory T cells is increased in peripheral blood and tumor microenvironment of patients with pancreas or breast adenocarcinoma [J]. J Immunol, 2002, 169(5): 27562761.
  10. 10. Hiraoka N, Onozato K, Kosuge T, et al. Prevalence of FOXP3+ regulatory T cells increases during the progression of pancreatic ductal adenocarcinoma and its premalignant lesions [J]. Clin Cancer Res, 2006, 12(18): 54235434.
  11. 11. Kono K, Kawaida H, Takahashi A, et al. CD4+CD25high regulatory T cells increase with tumor stage in patient with gastric and esophageal cancers [J]. Cancer Immunol Immunother, 2006, 55(9): 10641071.
  12. 12. Peng L, Kiaergard J, Plautz GE, et al. Tumorinduced Lselectin high suppressor T cells mediate potent effector T cell blockade and cause failure of otherwise curative adoptive immunotherapy [J]. J Immunol, 2002, 169(9): 48114821.
  13. 13. 鄔玉輝, 申正堂, 海健, 等. 乳腺癌患者細胞免疫功能的檢測 [J]. 中國醫(yī)師雜志, 2001, 3(8): 627628.
  14. 14. Sharma S, Yang SC, Zhu L, et al. Tumor cyclooxygenase2/prostaglandin E2dependent promotion of FOXP3 expression and CD4+CD25+ T regulatory cell activities in lung cancer [J]. Cancer Res, 2005, 65(12): 52115220.
  15. 15. Valzasina B, Piconese S, Guiducci C, et al. Tumorinduced expansion of regulatory T cells by conversion of CD4+CD25- lymphocytes is thymus and proliferation independent [J]. Cancer Res, 2006, 66(8): 44884495.
  16. 16. Ghiringhelli F, Puig PE, Roux S, et al. Tumor cells conver immature myeloid dendritic cells into TGFβsecreting cells inducing CD4+CD25+ regulatory T cell proliferation [J]. J Exp Med, 2005, 202(7): 919929.
  17. 17. MooYoung TA, Larson JW, Belt BA, et al. Tumorderived TGFβ mediates conversion of CD4+Foxp3+ regulatory T Cells in a murine model of pancreas cancer [J]. J Immunother, 2009, 32(1): 1221.
  18. 18. Curiel J, Conkos G, Zou L, et al. Specifc recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival [J]. Nat Med, 2004,10(9): 942949.
  19. 19. Dannull J, Su Z, Rizzieri D, et al. Enhancement of vaccinemediated antitumor immunity in cancer patients after depletion of regulatory T cells [J]. J Clin Invest, 2005, 115(12): 36233633.
  20. 20. Nummer D, SuriPayer E, SchmitzWinnenthal H, et al. Role of tumor endothelium in CD4+CD25+ regulatory T cell infiltration of human pancreatic carcinoma [J]. J Natl Cancer Inst, 2007, 99(15): 11881199.
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