目的：探討雌激素影響人精子頂體反應的可能機制。方法：應用異硫氰酸熒光素標記豌豆凝集素熒光染色法（FITC-PSA）分析精子頂體反應（AR）、以分光光度比色法測定頂體酶（ACE）活性。結果：17β-雌二醇（17β-E2）可促進精子發(fā)生AR，并增強精子頂體酶的活性；去除培養(yǎng)液中的Ca2+后，17β-E2不能誘導精子發(fā)生AR；PKC抑制劑能明顯降低17β-E2所誘導的AR；E2-BSA亦能夠促進精子發(fā)生AR，其作用與17β-E2無顯著差異。結論：雌激素對人精子頂體反應有一定的促進作用，增強精子頂體酶活性可能是其作用途徑之一，此過程涉及了胞外Ca2+、PKC及精子膜上的ER或雌激素結合位點的參與。
引用本文： 何彥芳,鄭潔,岳利民,何亞平,張金虎,王 嶸. 17β-雌二醇影響人精子頂體反應機制的初步研究. 華西醫(yī)學, 2008, 23(1): 34-35. doi: 復制
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- 3. Revelli A,Costamagna C,Mofa F,et a1.Signaling pathway of nitric oxide-induced acrosome reaction in human spermatozoa[J].Biol Reprod,2001,64(6):1708-1712.
- 4. 肖春花，王忠山，左文靜，等．精子頂體酶活性檢測新方法與臨床應用[J]．男性學雜志，1994，8(4)：98-200.
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- 6. Cui YH,Zhao RL,Wang Q,et a1.Determination of sperm acrosin activity for evaluation of male fertility[J].Asian J Andm1,2000,2(3):229-232.
- 7. Breitbart H.Intracellular calcium regulation in sperm capacitation and acrosomal reaction[J].Mol Cell Endocrinol,2002,187(1-2):139-144.
- 8. Patrat C,Serresc C,Jounnet P.The aerosome reaction in human spermatozoa.Biol Cell,2000,92(3-4):255-266.
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