• 1.四川遂寧市人民醫(yī)院神經(jīng)內(nèi)科，四川遂寧 629000;2.四川大學(xué)華西醫(yī)院神經(jīng)內(nèi)科，四川成都 610041;

目的:β淀粉樣蛋白（β-amyloid precursor protein，β-APP）是已知的參與阿爾茨海默病機(jī)制的關(guān)鍵因子。β-APP是否參與難治性癲癇中的病理機(jī)制并不清楚。這項(xiàng)研究在于了解β-APP的蛋白在難治性癲癇患者術(shù)后顳葉皮質(zhì)和海馬組織中的表達(dá)是否異常。方法:免疫熒光法半定量測定難治性癲癇患者術(shù)后顳葉皮質(zhì)和海馬組織中的β-APP陽性蛋白的熒光值，并應(yīng)用統(tǒng)計(jì)軟件對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行單因素方差分析。結(jié)果:免疫熒光強(qiáng)度值分析結(jié)果顯示β-APP在耐藥性癲癇腦組織中表達(dá)較對(duì)照組明顯增高且有統(tǒng)計(jì)學(xué)意義。結(jié)論:β-APP在難治性癲癇腦組織中異常增高，增高的β-APP可能參與了難治性癲癇的病理機(jī)制。

引用本文： 蔣世杰,楊小芳,李勁梅. 免疫熒光和免疫組化聯(lián)合檢測難治性癲癇患者腦組織中β類淀粉蛋白的表達(dá). 華西醫(yī)學(xué), 2009, 24(1): 32-34. doi: 復(fù)制

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11. Hoshi M，Sato M，Matsumoto S，et al.Spherical aggregates of βamyloid （amylospheroid） show high neurotoxicity and activate tau protein kinase Ⅰ/glycogen synthase kinase3β[J].Proc Natl Acad Sci USA，2003，100（11）:6370-6375..
12. Han P，Dou F，Li F，et al.Suppression of cyclindependent kinase 5 activation by amyloid precursor protein:a novel excitoprotective mechanism involving modulation of tau phosphorylation[J].J Neurosci，2005，25（50）:11542-11552..
13. Takashima A，Honda T，Yasutake K，et al.Activation of tau protein kinase Ⅰ/glycogen synthase kinase3β by amyloid β peptide （2535） enhances phosphorylation of tau in hippocampal neurons[J].Neurosci Res，1998，31（4）:317-323..
14. Lee MS，Kwon YT，Li M，et al.Neurotoxicity induces cleavage of p35 to p25 by calpain[J].Nature，2000，405（6784）:360-364..
15. Greenberg SM，Koo EH，Selkoe DJ，et al.Secreted βamyloid precursor protein stimulates mitogenactivated protein kinase and enhances tau phosphorylation[J].Proc Natl Acad Sci USA，1994，91（15）:7104-7108.
  1. 1. Salinero O，MorenoFlores MT，Wandosell F.Increasing neurite outgrowth capacity of βamyloid precursor protein proteoglycan in Alzheimer’s disease[J].J Neurosci Res，2000，60（1）:87-97..
  2. 2. 吳遜，沈鼎烈.難治性癲癇[J].中華神經(jīng)科雜志，1998，31:4..
  3. 3. 王學(xué)峰，肖波，孫紅斌.難治性癲癇[M].上?？茖W(xué)技術(shù)出版社，2001:5-11..
  4. 4. Perez RG，Zheng H，Van der Ploeg LH，et al.The βamyloid precursor protein of Alzheimer’s disease enhances neuron viability and modulates neuronal polarity[J].J Neurosci，1997，17（24）:9407-9414..
  5. 5. Niess C，Grauel U，Toennes SW，et al.Incidence of axonal injury in human brain tissue[J].Acta Neuropathol（Berl），2002，104（1）:79-84..
  6. 6. Smith DH，Chen XH，Iwata A，et al.Amyloid β accumulation in axons after traumatic brain injury in humans[J].J Neurosurg，2003，98（5）:1072-1077..
  7. 7. GordonKrajcer W，Gajkowska B.Excitotoxicityinduced expression of amyloid precursor protein （βAPP） in the hippocampus and cortex of rat brain.An electronmicroscopy and biochemical study[J].Folia Neuropathol，2001，39（3）:163-173..
  8. 8. 汪建華，王學(xué)峰，龔云，等.耐藥性癲癇患者術(shù)后腦組織中Tau蛋白的表達(dá)[J].中華神經(jīng)科雜志，2005，38（9）:543-546..
  9. 9. Tomizawa K，Cai XH，Moriwaki A，et al.Involvement of cyclindependent kinase 5/p35（nck5a） in the synaptic reorganization of rat hippocampus during kindling progression[J].Jpn J Physiol，2000，50（5）:525-532.
  10. 10. Pollard H，Khrestchatisky M，Moreau J，et al.Correlation between reactive sprouting and microtubule protein expression in epileptic hippocampus[J].Nuroscience，1994，63（2）:627..
  11. 11. Hoshi M，Sato M，Matsumoto S，et al.Spherical aggregates of βamyloid （amylospheroid） show high neurotoxicity and activate tau protein kinase Ⅰ/glycogen synthase kinase3β[J].Proc Natl Acad Sci USA，2003，100（11）:6370-6375..
  12. 12. Han P，Dou F，Li F，et al.Suppression of cyclindependent kinase 5 activation by amyloid precursor protein:a novel excitoprotective mechanism involving modulation of tau phosphorylation[J].J Neurosci，2005，25（50）:11542-11552..
  13. 13. Takashima A，Honda T，Yasutake K，et al.Activation of tau protein kinase Ⅰ/glycogen synthase kinase3β by amyloid β peptide （2535） enhances phosphorylation of tau in hippocampal neurons[J].Neurosci Res，1998，31（4）:317-323..
  14. 14. Lee MS，Kwon YT，Li M，et al.Neurotoxicity induces cleavage of p35 to p25 by calpain[J].Nature，2000，405（6784）:360-364..
  15. 15. Greenberg SM，Koo EH，Selkoe DJ，et al.Secreted βamyloid precursor protein stimulates mitogenactivated protein kinase and enhances tau phosphorylation[J].Proc Natl Acad Sci USA，1994，91（15）:7104-7108.