抗原B ELISA法对细粒棘球蚴病诊断价值的Meta分析_《中国循证医学杂志》

作者：

李艳红 , 孟存仁 ,  张朝霞

新疆医科大学第一附属医院医学检验中心（乌鲁木齐 830054）;

关键词：

细粒棘球蚴病 AgB 酶联免疫吸附试验 Meta分析诊断性试验

DOI：

10.7507/1672-2531.20130057

视频：

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摘要 全文 图表 视频 参考文献 施引文献 补充材料

目的　系统评价抗原B ELISA（AgB ELISA）法对细粒棘球蚴病的诊断价值。
方法　计算机检索PubMed、EMbase、The Cochrane Library、EBSCO、CBM、CNKI、WanFang Data和超星Medalink数据库，并手工检索相关期刊，全面收集AgB ELISA法诊断细粒棘球蚴病的诊断性试验，检索时限从建库至2012年7月5日。由2位研究者按纳入与排除独立筛选文献、提取资料并按QUADAS标准评价纳入研究的方法学质量后，采用Meta-Disc 1.4软件进行Meta分析。
结果　最终纳入8个研究，包括562例经金标准确诊的细粒棘球蚴病患者，434例相似病例和303例健康人。8个研究间无阈值效应（灵敏度对数与1-特异度对数的Spearman相关系数=0.527，P=0.400 1），采用随机效应模型进行Meta分析结果显示，合并灵敏度、特异度、阳性似然比、阴性似然比及诊断比值比分别为0.76［95%CI（0.73，0.79）］、0.84［95%CI（0.82，0.86）］、5.20［95%CI（3.59，7.55）］、0.26［95%CI（0.18，0.35）］、23.93［95%CI（12.35，46.39）］，SROC曲线下面积（AUC）=0.889 7，Q=0.820 4。
结论　采用天然AgB和rAgB ELISA法检测细粒棘球蚴病具有较大诊断价值。

引用本文： 李艳红,孟存仁,张朝霞. 抗原B ELISA法对细粒棘球蚴病诊断价值的Meta分析. 中国循证医学杂志, 2013, 13(3): 332-338. doi: 10.7507/1672-2531.20130057 复制

1. 刘洪波, 谢妍, 王宁. 包虫病. 中国实用乡村医生杂志, 2006, 13(11): 10-11.

2. Musiani P, Piantelli M, Lauriola L, et al. Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids. J Clin Pathol, 1978, 31(5): 475-478.

3. HaAg KL, Zanotto PM, Alves-Junior L, et al. Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infect Genet Evol, 2006, 6(4): 251-261.

4. HaAg KL, Gott stein B, Muller N, et al. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitol, 2006, 133(4): 411-419.

5. Mamuti W, Sako Y, Bart JM, et al. Molecular characterization of anovelgeneencodingan8kDasubunit of antigen B from Echinococcus granulosus genotypes 1 and 6. Parasitol Int, 2007, 56(2): 313-316.

6. Parija SC. A review of some simple immunoassays in the serodiAgnosis of cystic hydatid disease. Acta Trop, 1998, 70(1): 17-24.

7. Paul M, Stefaniak J. Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diAgnosis of liver cystic echinococcosis. Hepatol Res, 2001, 21(1): 14-26.

8. Whiting P, Rutjes AW, Reitsma JB, 等. QUADAS的制定: 用于系统评价中评价诊断性研究质量的工具. 中国循证医学杂志, 2007, 7(4): 296-306.

9. Whiting P, Weswood ME, Rutjes AW, 等. QUADAS评价: 一种用于诊断性研究的质量评价工具(修订版). 中国循证医学杂志, 2007, 7(7): 531-536.

10. Sbihi Y, Rmiqui A, Rodriguez-Cabezas MN, et al. Comparative sensitivity of six serological tests and diAgnostic value of ELISA using purified antigen in hydatidosis. J Clin Lab Anal, 2001, 15(1): 14-18.

11. Sadjjadi SM, Abidi H, Sarkari B, et al. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiAgnosis of human hydatidosis. Iran J Immunol, 2007, 4(3): 167-172.

12. González-Sapienza G, Lorenzo C, Nieto A. Improved immunodiAgnosis of cystic hydatid disease by using a synthetic peptide with higher diAgnostic value than that of its parent protein, Echinococcus granulosus antigen B. J Clin Microbiol, 2000, 38(11): 3979-3983.

13. Kalantari E, Bandehpour M, Pazoki R, et al. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diAgnosing hydatidosis. Parasitol Res, 2010, 106(4): 847-851.

14. 江莉, 冯正, 许学年. 细粒棘球蚴AgB亚单位基因的联合表达和血清学评价. 热带医学杂志, 2009, 9(1): 25-41.

15. Jiang L, Zhang YG, Liu MX, et al. Analysis on the reactivity of five subunits of antigen B family in serodiAgnosis of echinococcosis. Exp Parasitol, 2012, 131(1): 85-91.

16. 高春花, 崔刚, 王俊云, 等. 我国细粒棘球绦虫新疆分离柱AgB8/3亚基克隆表达及其检测囊型包虫病价值分析. 中国人兽共患病学报, 2011, 27(11): 966-969.

17. 马秀敏, 乌拉木·马木提, 马海梅, 等. 细粒棘球蚴重组AgB8-kDa 亚单位1对囊型包虫病的血清学诊断价值. 中国人兽共患病学报, 2009, 25(8), 741-744.

18. Hadgu A. The discrepancy in discrepant analysis. Lancet, 1996, 348(9027): 592-593.

19. Kato I, Santamaria M, De Ruiz PA, et al. Inter-observer variation in cytological and histological diAgnoses of cervical neoplasia and its epidemiologic implication. J Clin Epidemiol, 1995, 48(9): 1167-1174.

20. Tackmann R, Schuetz G, Hamm B, et al. Quality of the reporting of diAgnostic accuracy studies: STARD (Standards for the Reporting of DiAgnostic accuracy studies). Rofo, 2010, 182(8): 655-659.

21. 秦亮, 移康, 马彬, 等. 抗角蛋白抗体对类风湿性关节炎诊断价值的系统评价. 中国循证医学杂志, 2011, 11(3): 265 -272.

1. 刘洪波, 谢妍, 王宁. 包虫病. 中国实用乡村医生杂志, 2006, 13(11): 10-11.

2. Musiani P, Piantelli M, Lauriola L, et al. Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids. J Clin Pathol, 1978, 31(5): 475-478.

3. HaAg KL, Zanotto PM, Alves-Junior L, et al. Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infect Genet Evol, 2006, 6(4): 251-261.

4. HaAg KL, Gott stein B, Muller N, et al. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitol, 2006, 133(4): 411-419.

5. Mamuti W, Sako Y, Bart JM, et al. Molecular characterization of anovelgeneencodingan8kDasubunit of antigen B from Echinococcus granulosus genotypes 1 and 6. Parasitol Int, 2007, 56(2): 313-316.

6. Parija SC. A review of some simple immunoassays in the serodiAgnosis of cystic hydatid disease. Acta Trop, 1998, 70(1): 17-24.

7. Paul M, Stefaniak J. Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diAgnosis of liver cystic echinococcosis. Hepatol Res, 2001, 21(1): 14-26.

8. Whiting P, Rutjes AW, Reitsma JB, 等. QUADAS的制定: 用于系统评价中评价诊断性研究质量的工具. 中国循证医学杂志, 2007, 7(4): 296-306.

9. Whiting P, Weswood ME, Rutjes AW, 等. QUADAS评价: 一种用于诊断性研究的质量评价工具(修订版). 中国循证医学杂志, 2007, 7(7): 531-536.

10. Sbihi Y, Rmiqui A, Rodriguez-Cabezas MN, et al. Comparative sensitivity of six serological tests and diAgnostic value of ELISA using purified antigen in hydatidosis. J Clin Lab Anal, 2001, 15(1): 14-18.

11. Sadjjadi SM, Abidi H, Sarkari B, et al. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiAgnosis of human hydatidosis. Iran J Immunol, 2007, 4(3): 167-172.

12. González-Sapienza G, Lorenzo C, Nieto A. Improved immunodiAgnosis of cystic hydatid disease by using a synthetic peptide with higher diAgnostic value than that of its parent protein, Echinococcus granulosus antigen B. J Clin Microbiol, 2000, 38(11): 3979-3983.

13. Kalantari E, Bandehpour M, Pazoki R, et al. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diAgnosing hydatidosis. Parasitol Res, 2010, 106(4): 847-851.

14. 江莉, 冯正, 许学年. 细粒棘球蚴AgB亚单位基因的联合表达和血清学评价. 热带医学杂志, 2009, 9(1): 25-41.

15. Jiang L, Zhang YG, Liu MX, et al. Analysis on the reactivity of five subunits of antigen B family in serodiAgnosis of echinococcosis. Exp Parasitol, 2012, 131(1): 85-91.

16. 高春花, 崔刚, 王俊云, 等. 我国细粒棘球绦虫新疆分离柱AgB8/3亚基克隆表达及其检测囊型包虫病价值分析. 中国人兽共患病学报, 2011, 27(11): 966-969.

17. 马秀敏, 乌拉木·马木提, 马海梅, 等. 细粒棘球蚴重组AgB8-kDa 亚单位1对囊型包虫病的血清学诊断价值. 中国人兽共患病学报, 2009, 25(8), 741-744.

18. Hadgu A. The discrepancy in discrepant analysis. Lancet, 1996, 348(9027): 592-593.

19. Kato I, Santamaria M, De Ruiz PA, et al. Inter-observer variation in cytological and histological diAgnoses of cervical neoplasia and its epidemiologic implication. J Clin Epidemiol, 1995, 48(9): 1167-1174.

20. Tackmann R, Schuetz G, Hamm B, et al. Quality of the reporting of diAgnostic accuracy studies: STARD (Standards for the Reporting of DiAgnostic accuracy studies). Rofo, 2010, 182(8): 655-659.

21. 秦亮, 移康, 马彬, 等. 抗角蛋白抗体对类风湿性关节炎诊断价值的系统评价. 中国循证医学杂志, 2011, 11(3): 265 -272.

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《中国循证医学杂志》

抗原B ELISA法对细粒棘球蚴病诊断价值的Meta分析

摘要 全文 图表 视频 参考文献 施引文献 补充材料

上一篇

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Content

1.	刘洪波, 谢妍, 王宁. 包虫病. 中国实用乡村医生杂志, 2006, 13(11): 10-11.
2.	Musiani P, Piantelli M, Lauriola L, et al. Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids. J Clin Pathol, 1978, 31(5): 475-478.
3.	HaAg KL, Zanotto PM, Alves-Junior L, et al. Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infect Genet Evol, 2006, 6(4): 251-261.
4.	HaAg KL, Gott stein B, Muller N, et al. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitol, 2006, 133(4): 411-419.
5.	Mamuti W, Sako Y, Bart JM, et al. Molecular characterization of anovelgeneencodingan8kDasubunit of antigen B from Echinococcus granulosus genotypes 1 and 6. Parasitol Int, 2007, 56(2): 313-316.
6.	Parija SC. A review of some simple immunoassays in the serodiAgnosis of cystic hydatid disease. Acta Trop, 1998, 70(1): 17-24.
7.	Paul M, Stefaniak J. Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diAgnosis of liver cystic echinococcosis. Hepatol Res, 2001, 21(1): 14-26.
8.	Whiting P, Rutjes AW, Reitsma JB, 等. QUADAS的制定: 用于系统评价中评价诊断性研究质量的工具. 中国循证医学杂志, 2007, 7(4): 296-306.
9.	Whiting P, Weswood ME, Rutjes AW, 等. QUADAS评价: 一种用于诊断性研究的质量评价工具(修订版). 中国循证医学杂志, 2007, 7(7): 531-536.
10.	Sbihi Y, Rmiqui A, Rodriguez-Cabezas MN, et al. Comparative sensitivity of six serological tests and diAgnostic value of ELISA using purified antigen in hydatidosis. J Clin Lab Anal, 2001, 15(1): 14-18.
11.	Sadjjadi SM, Abidi H, Sarkari B, et al. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiAgnosis of human hydatidosis. Iran J Immunol, 2007, 4(3): 167-172.
12.	González-Sapienza G, Lorenzo C, Nieto A. Improved immunodiAgnosis of cystic hydatid disease by using a synthetic peptide with higher diAgnostic value than that of its parent protein, Echinococcus granulosus antigen B. J Clin Microbiol, 2000, 38(11): 3979-3983.
13.	Kalantari E, Bandehpour M, Pazoki R, et al. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diAgnosing hydatidosis. Parasitol Res, 2010, 106(4): 847-851.
14.	江莉, 冯正, 许学年. 细粒棘球蚴AgB亚单位基因的联合表达和血清学评价. 热带医学杂志, 2009, 9(1): 25-41.
15.	Jiang L, Zhang YG, Liu MX, et al. Analysis on the reactivity of five subunits of antigen B family in serodiAgnosis of echinococcosis. Exp Parasitol, 2012, 131(1): 85-91.
16.	高春花, 崔刚, 王俊云, 等. 我国细粒棘球绦虫新疆分离柱AgB8/3亚基克隆表达及其检测囊型包虫病价值分析. 中国人兽共患病学报, 2011, 27(11): 966-969.
17.	马秀敏, 乌拉木·马木提, 马海梅, 等. 细粒棘球蚴重组AgB8-kDa 亚单位1对囊型包虫病的血清学诊断价值. 中国人兽共患病学报, 2009, 25(8), 741-744.
18.	Hadgu A. The discrepancy in discrepant analysis. Lancet, 1996, 348(9027): 592-593.
19.	Kato I, Santamaria M, De Ruiz PA, et al. Inter-observer variation in cytological and histological diAgnoses of cervical neoplasia and its epidemiologic implication. J Clin Epidemiol, 1995, 48(9): 1167-1174.
20.	Tackmann R, Schuetz G, Hamm B, et al. Quality of the reporting of diAgnostic accuracy studies: STARD (Standards for the Reporting of DiAgnostic accuracy studies). Rofo, 2010, 182(8): 655-659.
21.	秦亮, 移康, 马彬, 等. 抗角蛋白抗体对类风湿性关节炎诊断价值的系统评价. 中国循证医学杂志, 2011, 11(3): 265 -272.

《中国循证医学杂志》

抗原B ELISA法对细粒棘球蚴病诊断价值的Meta分析

摘要 全文 图表 视频 参考文献 施引文献 补充材料

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下一篇

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